氧化铁纳米颗粒(Fe3O4NPs)由于其在各个领域的不同应用而受到广泛关注。然而,人们还担心它们对环境和生物体的潜在毒性影响。在这项研究中,我们合成并表征了Fe3O4NPs,并评估了它们对fetida爱氏球菌的腔体细胞的免疫毒性。使用共沉淀法合成了Fe3O4NP,并使用X射线衍射(XRD)等技术测定了它们的物理化学性质,扫描电子显微镜-能量色散X射线(SEM-EDX),透射电子显微镜(TEM)和傅里叶变换红外光谱(FTIR)。合成的Fe3O4NP表现出具有球形形态的均匀尺寸分布,并且从XRD分析证实了相纯度。为了评估Fe3O4NPs的免疫毒性,将fitida的Eisenia结肠细胞暴露于各种浓度的Fe3O4NP中14天。此外,我们分析了Fe3O4NPs对生化参数的影响,包括超氧化物歧化酶(SOD),过氧化氢酶(CAT),酸性磷酸酶(AP),碱性磷酸酶(ALP),和总蛋白质含量(TPC),并进行了组织学检查。生化分析显示SOD活性水平显著改变,CAT,AP,ALP,和腔体细胞中的TPC,表明暴露于Fe3O4NPs后免疫系统失调。此外,组织学检查显示结构变化,提示由Fe3O4NP引起的细胞损伤。这些发现提供了有关Fe3O4NPs对费蒂达艾森亚的免疫毒性作用的有价值的见解,并强调需要进一步研究纳米颗粒对环境的潜在影响。
Iron oxide nanoparticles (Fe3O4 NPs) have gained considerable attention due to their diverse applications in various fields. However, concerns about their potential toxic effects on the environment and living organisms have also emerged. In this study, we synthesized and characterized Fe3O4 NPs and assessed their
immunotoxicity on the coelomocytes of Eisenia fetida. The Fe3O4 NPs were synthesized using a co-precipitation method, and their physicochemical properties were determined using techniques such as X-ray diffraction (XRD), scanning electron microscopy-energy dispersive X-ray (SEM-EDX), transmission electron microscopy (TEM) and Fourier-transform infrared spectroscopy (FTIR). The synthesized Fe3O4 NPs exhibited a uniform size distribution with spherical morphology and the phase purity was confirmed from XRD analysis. To evaluate the
immunotoxicity of Fe3O4 NPs, Eisenia fetida coelomocytes were exposed to various concentrations of Fe3O4 NPs for 14 days. Furthermore, we analyzed the impact of Fe3O4 NPs on the biochemical parameters, including superoxide dismutase (SOD), catalase (CAT), acid phosphatase (APs), alkaline phosphatase (ALP), and total protein content (TPC), as well as conducted a histological examination. Biochemical analysis revealed significant alterations in the activity levels of SOD, CAT, APs, ALP, and TPC in the coelomocytes, indicating immune system dysregulation upon exposure to Fe3O4 NPs. Moreover, histological examination demonstrated structural changes, suggesting cellular damage caused by Fe3O4 NPs. These findings provide valuable insights into the immunotoxic effects of Fe3O4 NPs on Eisenia fetida and underscore the need for further investigation into the potential environmental impact of nanoparticles.