目的:通过免疫组织化学方法研究死袋综合征(DBS)患者晶状体囊中的细胞外基质和细胞成分。
方法:眼科,和歌山医科大学医学院,眼科和视觉科学系,约翰·莫兰眼科中心,犹他大学。
方法:免疫组化实验研究。
方法:来自DBS病例的9个囊袋标本,以及2个对照标本来自与先前玻璃体切除术相关的术后晚期袋内人工晶状体脱位病例,假性剥脱,钝性外伤也包括在内.对它们进行组织病理学处理;从每个样本中获得未染色的切片,并通过免疫组织化学靶向IV型胶原进行分析,层粘连蛋白,波形蛋白,I型胶原和纤连蛋白。
结果:DBS的免疫组织化学显示晶状体囊基底膜成分染色。与后部相比,从内部分裂的前囊的外部对IV型胶原蛋白的染色更明显。纤维后囊混浊(PCO)成分的模糊染色,e.g.,I型胶原和纤连蛋白,在有限的区域被发现,但是胶囊的主要部分没有这些成分。小斑点波形蛋白阳性材料,表明细胞碎片的存在,在有限的样本中也检测到。
结论:从DBS患者中提取的胶囊中检测到少量纤维化PCO成分,但它们的主要部件没有PCO组件。目前的发现表明,手术后存在少量晶状体上皮细胞,并在经历细胞死亡过程之前分泌纤维成分。
OBJECTIVE: To investigate the extracellular matrix and cellular components in lens capsules extracted from patients with dead bag syndrome (DBS) through immunohistochemistry.
METHODS: Department of Ophthalmology, Wakayama Medical University School of Medicine, Wakayama, Japan, and Department of Ophthalmology and Visual Sciences, John A. Moran Eye Center, University of Utah, Salt Lake City, Utah.
METHODS: Immunohistochemical experimental study.
METHODS: 9 capsular bag specimens from DBS cases, as well as 2 control specimens from late-postoperative in-the-bag intraocular lens dislocation cases related to previous vitrectomy, pseudoexfoliation, and blunt trauma were included. They were processed for histopathology; unstained sections were obtained from each one and analyzed by immunohistochemistry targeting collagen type IV, laminin, vimentin, collagen type I, and fibronectin.
RESULTS: Immunohistochemistry in DBS showed lens capsule stained for basement membrane components. The outer part of the anterior capsule that was split from the inner part was more markedly stained for type IV collagen as compared with the posterior part. Faint staining for fibrous posterior capsular opacification (PCO) components, for example, collagen type I and fibronectin, was detected in limited areas, but the major portion of the capsule was free from these components. Small spotty vimentin-positive materials, suggesting the presence of cell debris, were also detected in limited samples.
CONCLUSIONS: Small amounts of fibrotic PCO components were detected in capsules extracted from patients with DBS, but their major parts were free from PCO components. Current findings suggest small amounts of lens epithelial cells were present after surgery and secreted fibrous components before undergoing cell death process.