■除了对COVID-19病毒的临床和实验室研究之外,COVID-19免疫遗传学领域的生物信息学研究尤为重要。在这个账户中,研究表明,在COVID-19患者中,肿瘤坏死α(TNFα)和白细胞介素-6(IL-6)的水平很高,在COVID-19的严重病例中,IL-6,TNF-α的产生,和其他细胞因子急剧增加。另一方面,研究IL-6和TNFα的分子结构和受体以及受体蛋白的结构分析可能有助于开发新的COVID-19感染治疗方案。
■为了在包含来自GEO的转录谱的微阵列数据集中鉴定COVID-19患者中具有显著和不同表达的基因作为功能基因组数据库,下载了编程语言R版本4.2.1的GEO查询软件包版本2.64.2。这样,DEGs的功能富集分析,WikiPathways,REGO,基因本体论,和STRING数据库也进行了调查和使用。
■研究了参与COVID-19发病的促炎细胞因子TNFα和IL-6的结构和功能,总的来说,在本研究中进行了各种分析,并从KEGG数据库中提取了一系列具有不同表达的基因,最后5个DEGs包括CXCL14、CXCL6、CCL8、CXCR1、TNFRSF10,并观察了它们在不同通路中的关系和表达效果。
■IL-6和TNFα参与免疫过程,与细胞因子的激活有直接和间接的关系,包括IL6和TNF-a,和细胞因子风暴,这表明它们在问题和并发症形成中的作用,包括ARDS,在COVID-19患者中。当然,确定这些基因的有效性需要更专业和临床研究。
UNASSIGNED: Besides the clinical and laboratory research on the COVID-19 virus, the bioinformatics study in the field of genetics of immunity to COVID-19 is of particular importance. In this account, studies show that in patients with COVID-19, the level of tumor necrosis alpha (TNFα) and interleukin-6 (IL-6) is high and in severe cases of COVID-19, the production of IL-6, TNF-α, and other cytokines increases profoundly. On the other hand, investigating the molecular structure and receptors of IL-6 and TNFα and the structural analysis of the receptor proteins may potentially help to develop new therapeutic plans for COVID-19 infection.
UNASSIGNED: To identify genes with significant and different expressions in patients with COVID-19 in a microarray data set containing transcriptional profiles from GEO as a functional genomic database the GEO query package version 2.64.2 in a programming language R version 4.2.1 was downloaded. In this way, functional enrichment analysis for DEGs, WikiPathways, REGO, gene ontology, and STRING database was also investigated and employed.
UNASSIGNED: The structure and function of pro-inflammatory cytokines TNFα and IL-6 involved in the pathogenesis of COVID-19 were investigated, and in general, after performing various analyses in this study and extracting A series of genes with different expressions from the KEGG database, the final 5 DEGs include CXCL14, CXCL6, CCL8, CXCR1, TNFRSF10, and the relationship and expression effects of them were observed in different pathways.
UNASSIGNED: IL-6 and TNFα were involved in immunological processes that had a direct and indirect relationship with the activation of cytokines, including IL6 and TNF-a, and cytokine storm, and this indicates their role in the formation of problems and complications, including ARDS, in COVID-19 patients. Of course, determining the effectiveness of each of these genes requires more specialized and clinical studies.