未经证实:免疫球蛋白A(IgA)是体液中的主要抗体同种型,例如眼泪,肠粘液,初乳,还有唾液.人类中IgA有两种亚型:IgA1,主要存在于血液和粘膜部位,和IgA2,优先表达在粘膜部位,如结肠。在临床实践中,免疫球蛋白通常在静脉或毛细血管血液中测量;然而,替代样品,包括唾液,现在正在考虑,鉴于它们的非侵入性和易于收集的性质。几种自身免疫性疾病与口腔粘膜免疫的各种异常有关,比如类风湿性关节炎,干燥综合征,和系统性红斑狼疮(SLE)。
UNASSIGNED:我们决定评估SLE患者唾液中两种IgA亚型的水平。与年龄和性别匹配的健康志愿者相比,基于轻链捕获的ELISA测量了一组SLE患者中的特异性IgA1和IgA2水平。
未经批准:令人惊讶的是,我们的结果表明,在SLE患者的唾液中,总IgA和IgA1亚型显著升高;我们还发现,特别是IgA2,与抗dsDNAIgG抗体滴度呈正相关。引人注目的是,我们还检测到SLE患者唾液抗核小体IgA抗体的存在,以前未在其他地方报告的功能。
未经评估:根据我们的结果和必要的验证,唾液中的IgA表征可以代表SLE患者临床护理中的潜在有用工具,具有更直接的优势,更快,比操作血液样本更安全的方法。
Immunoglobulin A (IgA) is the main antibody isotype in body fluids such as tears, intestinal mucous, colostrum, and saliva. There are two subtypes of IgA in humans: IgA1, mainly present in blood and mucosal sites, and
IgA2, preferentially expressed in mucosal sites like the colon. In clinical practice, immunoglobulins are typically measured in venous or capillary blood; however, alternative samples, including saliva, are now being considered, given their non-invasive and easy collection nature. Several autoimmune diseases have been related to diverse abnormalities in oral mucosal immunity, such as rheumatoid arthritis, Sjogren\'s syndrome, and systemic lupus erythematosus (SLE).
We decided to evaluate the levels of both IgA subtypes in the saliva of SLE patients. A light chain capture-based ELISA measured specific IgA1 and
IgA2 levels in a cohort of SLE patients compared with age and gender-matched healthy volunteers.
Surprisingly, our results indicated that in the saliva of SLE patients, total IgA and IgA1 subtype were significantly elevated; we also found that salivary IgA levels, particularly
IgA2, positively correlate with anti-dsDNA IgG antibody titers. Strikingly, we also detected the presence of salivary anti-nucleosome IgA antibodies in SLE patients, a feature not previously reported elsewhere.
According to our results and upon necessary validation, IgA characterization in saliva could represent a potentially helpful tool in the clinical care of SLE patients with the advantage of being a more straightforward, faster, and safer method than manipulating blood samples.