蛇头囊泡病毒(SHVV)是一种负义单链RNA病毒,可感染蛇头鱼。这种病毒导致疾病和死亡,给蛇头养殖业造成重大的经济损失。SHVV在细胞中的复制和传播,需要谷氨酰胺作为氮源,伴随着细胞内代谢物的改变。然而,谷氨酰胺缺乏抑制病毒复制的代谢机制尚不清楚.这项研究利用液相色谱-质谱法测量了在含谷氨酰胺和缺乏谷氨酰胺的条件下感染SHVV的CatfishParasilurusasotus卵巢细胞系之间的差异代谢物。结果表明,谷氨酰胺的缺乏调节了4种不同的代谢途径,并影响了9种差异代谢产物。差异代谢物PS(16:0/16:0),5,10-亚甲基-THF,PS(18:0/18:1(9Z))参与氨基酸代谢。在核代谢功能途径中,观察到鸟苷的差异代谢产物。在碳水化合物代谢途径中,检测UDP-d-半乳糖醛酸的差异代谢产物。在信号转导通路中,SM的差异代谢物(d18:1/20:0),SM(d18:1/22:1(13Z)),SM(d18:1/24:1(15Z)),发现了鞘氨醇。其中,PS(18:0/18:1(9Z)),PS(16:0/16:0),UDP-d-半乳糖醛酸参与磷脂酰丝氨酸和糖蛋白的合成。化合物5,10-亚甲基-THF为病毒复制提供了原料,鸟苷和鞘氨醇与病毒毒力有关。差异代谢物可以共同参与复制,包装,谷氨酰胺缺乏和SHVV的增殖。本研究通过代谢组学方法为打击水产养殖中的SHVV感染提供了新的见解和潜在的代谢靶标。
Snakehead vesiculovirus (SHVV) is a negative-sense single-stranded RNA virus that infects snakehead fish. This virus leads to illness and mortality, causing significant economic losses in the snakehead aquaculture industry. The replication and spread of SHVV in cells, which requires glutamine as a nitrogen source, is accompanied by alterations in intracellular metabolites. However, the metabolic mechanisms underlying the inhibition of viral replication by glutamine deficiency are poorly understood. This study utilized liquid chromatography-mass spectrometry to measure the differential metabolites between the channel catfish Parasilurus asotus ovary cell line infected with SHVV under glutamine-containing and glutamine-deprived conditions. Results showed that the absence of glutamine regulated 4 distinct metabolic pathways and influenced 9 differential metabolites. The differential metabolites PS(16:0/16:0), 5,10-methylene-THF, and PS(18:0/18:1(9Z)) were involved in amino acid metabolism. In the nuclear metabolism functional pathway, differential metabolites of guanosine were observed. In the carbohydrate metabolism pathway, differential metabolites of UDP-d-galacturonate were detected. In the signal transduction pathway, differential metabolites of SM(d18:1/20:0), SM(d18:1/22:1(13Z)), SM(d18:1/24:1(15 Z)), and sphinganine were found. Among them, PS(18:0/18:1(9Z)), PS(16:0/16:0), and UDP-d-galacturonate were involved in the synthesis of phosphatidylserine and glycoprotein. The compound 5,10-methylene-THF provided raw materials for virus replication, and guanosine and sphingosine are related to virus virulence. The differential metabolites may collectively participate in the replication, packaging, and proliferation of SHVV under glutamine deficiency. This study provides new insights and potential metabolic targets for combating SHVV infection in aquaculture through metabolomics approaches.