可用于不同生物医学应用的天然存在的基于三维(3D)生物聚合物的基质是各种人造3D材料的可持续替代品。为此,来自海洋海绵的几丁质基结构是非常有前途的替代品。Verongiida(Demospongiae类)的海洋海绵是具有发育良好的几丁质骨骼的典型例子。特别是,属于Ianthellidae家族的物种具有几丁质,扁平,扇形纤维骨架具有独特的,微孔3D架构,使他们特别有趣的应用。在这项工作中,我们把注意力集中在假海绵上,1759)用于同时提取两种天然存在的(“即用型”)几丁质支架,和生物活性溴酪氨酸被认为是潜在的抗生素,抗肿瘤,和海洋防污物质。我们表明,选定的溴酪氨酸位于色素细胞内,然而,位于I.flabelliformis的几丁质骨骼纤维内。两步反应提供了两种产物:用甲醇处理萃取溴酪氨酸化合物bastadin25和araplysillin-IN20氨基磺酸盐,随后用乙酸和氢氧化钠处理暴露3D几丁质支架。这个脚手架是网状结构,保留了它的毛细血管网络,首次研究了其作为潜在药物递送生物材料的用途。结果表明,海绵衍生的几丁质支架,用德氨乙氧基浸渍,在琼脂扩散试验中有效抑制人病原体金黄色葡萄球菌的生长。
Naturally occurring three-dimensional (3D) biopolymer-based matrices that can be used in different biomedical applications are sustainable alternatives to various artificial 3D materials. For this purpose, chitin-based structures from marine sponges are very promising substitutes. Marine sponges from the order Verongiida (class Demospongiae) are typical examples of demosponges with well-developed chitinous skeletons. In particular, species belonging to the family Ianthellidae possess chitinous, flat, fan-like fibrous skeletons with a unique, microporous 3D architecture that makes them particularly interesting for applications. In this work, we focus our attention on the demosponge
Ianthella flabelliformis (Linnaeus, 1759) for simultaneous extraction of both naturally occurring (\"ready-to-use\") chitin scaffolds, and biologically active bromotyrosines which are recognized as potential antibiotic, antitumor, and marine antifouling substances. We show that selected bromotyrosines are located within pigmental cells which, however, are localized within chitinous skeletal fibers of I. flabelliformis. A two-step reaction provides two products: treatment with methanol extracts the bromotyrosine compounds bastadin 25 and araplysillin-I N20 sulfamate, and a subsequent treatment with acetic acid and sodium hydroxide exposes the 3D chitinous scaffold. This scaffold is a mesh-like structure, which retains its capillary network, and its use as a potential drug delivery biomaterial was examined for the first time. The results demonstrate that sponge-derived chitin scaffolds, impregnated with decamethoxine, effectively inhibit growth of the human pathogen Staphylococcus aureus in an agar diffusion assay.