有效地确定总纤维素酶活性而不降低准确性始终是一个挑战。最常见的总纤维素酶活性测定是由国际纯粹与应用化学联合会(IUPAC)建立的滤纸测定(FPA)。在这项工作中研究了一种使用基于微孔板的测定法测量FPA的新方法,遵循IUPAC稀释纤维素酶制备以获得固定葡萄糖释放的主要思想。用基于微孔板的测定法测定六种纤维素酶制剂的FPAs。结果表明,纤维素酶Youtell的FPAs,RCconc,R-10Lerkam,益水和国药分别为67.9、46.0、46.1、27.4、7.6和8.0IU/ml。用IUPAC测定的FPA和基于微孔板的测定在95%置信水平上没有显著差异。可以得出结论,与IUPAC相比,通过基于微孔板的测定可以以相同的精度和更高的效率确定FPA。
It is always a challenge to determine the total cellulase activity efficiently without reducing accuracy. The most common total cellulase activity assay is the filter paper assay (FPA) established by the International Union of Pure and Applied Chemistry (
IUPAC). A new procedure to measure the FPA with microplate-based assay was studied in this work, which followed the main idea of
IUPAC to dilute cellulase preparation to get fixed glucose release. FPAs of six cellulase preparations were determined with the microplate-based assay. It is shown that FPAs of cellulase Youtell, RCconc, R-10, Lerkam, Yishui and Sinopharm were 67.9, 46.0, 46.1, 27.4, 7.6 and 8.0 IU/ml respectively. There was no significant difference at the 95% confidence level between the FPA determined with
IUPAC and the microplate-based assay. It could be concluded that the FPA could be determined by the microplate-based assay with the same accuracy and much more efficiency compared with that by
IUPAC.