最近在武汉出现的疫情,中国于2019年12月在短时间内传播到整个世界,并杀死了>1,41万人。确定了一种称为严重急性呼吸道疾病冠状病毒2型(SARS-CoV-2)的新型β冠状病毒是这次爆发的病原体,由该病毒引起的疾病被命名为冠状病毒疾病19(COVID19)。尽管从病毒基因组结构中获得了信息,感染期间病毒与宿主相互作用的许多方面仍然未知.在这项研究中,我们旨在鉴定SARS-CoV-2编码的microRNAs及其细胞靶标。我们应用了一种计算方法来预测SARS-CoV-2编码的miRNA及其在人类中的假定靶标。预测的miRNA的靶标根据其生物过程聚集成组,分子功能,和使用GO和PANTHER的细胞隔室。通过使用KEGG途径富集分析,鉴定了顶部途径。最后,我们构建了一个与靶基因的整合途径网络分析。我们鉴定了40种SARS-CoV-2miRNAs及其调控靶标。我们的分析表明,靶向基因包括NFKB1,NFKBIE,JAK1-2,STAT3-4,STAT5B,STAT6,SOCS1-6,IL2,IL8,IL10,IL17,TGFBR1-2,SMAD2-4,HDAC1-6和JARID1A-C,JARID2在NFKB中起重要作用,JAK/STAT和TGFB信号通路以及细胞表观遗传调控通路。我们的结果可能有助于了解病毒与宿主的相互作用以及病毒miRNAs在SARS-CoV-2感染过程中的作用。由于目前尚无针对COVID19的药物和有效治疗方法,因此它也可能有助于开发新的治疗策略。
Recently an outbreak that emerged in Wuhan, China in December 2019, spread to the whole world in a short time and killed >1,410,000 people. It was determined that a new type of beta coronavirus called severe acute respiratory disease coronavirus type 2 (SARS-CoV-2) was causative agent of this outbreak and the disease caused by the virus was named as coronavirus disease 19 (COVID19). Despite the information obtained from the viral genome structure, many aspects of the virus-host interactions during infection is still unknown. In this study we aimed to identify SARS-CoV-2 encoded microRNAs and their cellular targets. We applied a computational method to predict miRNAs encoded by SARS-CoV-2 along with their putative targets in humans. Targets of predicted miRNAs were clustered into groups based on their biological processes, molecular function, and cellular compartments using GO and PANTHER. By using KEGG pathway enrichment analysis top pathways were identified. Finally, we have constructed an integrative pathway network analysis with target genes. We identified 40 SARS-CoV-2 miRNAs and their regulated targets. Our analysis showed that targeted genes including NFKB1, NFKBIE, JAK1-2, STAT3-4, STAT5B, STAT6, SOCS1-6, IL2, IL8, IL10, IL17, TGFBR1-2, SMAD2-4, HDAC1-6 and JARID1A-C, JARID2 play important roles in NFKB, JAK/STAT and TGFB signaling pathways as well as cells\' epigenetic regulation pathways. Our results may help to understand virus-host interaction and the role of viral miRNAs during SARS-CoV-2 infection. As there is no current drug and effective treatment available for COVID19, it may also help to develop new treatment strategies.