背景:这项研究的目的是比较龈沟液(GCF)的水平,唾液,和血清基质金属蛋白酶-9,白细胞介素(IL)-17,IL-36γ,和IL-38在健康牙周组织个体中,牙龈炎,和牙周炎,并评估其与临床牙周参数的相关性。
方法:90名全身健康且不吸烟的志愿者分为健康(H)组(n=30),牙龈炎(G)组(n=30),和牙周炎(P)组(n=30)纳入本研究。记录志愿者的临床牙周参数,和GCF,没有刺激的唾液,并收集血清样本。使用酶联免疫吸附测定进行数据分析。Kruskal-Wallis检验和Bonferroni校正用于多重比较和事后统计分析。
结果:H组的临床参数明显低于P组(p<0.001)。P组GCF和唾液IL-36γ、IL-38水平明显高于H组和G组(p<0.05)。观察到生化检查结果与临床牙周参数呈正相关。
结论:GCF中IL-36γ和IL-38水平,唾液,和血清与临床牙周参数相关,可能在确定牙周炎的活动中起作用。
The objective of this study was to compare the levels of gingival crevicular fluid (GCF), salivary, and serum matrix metalloproteinase-9, interleukin (IL)-17, IL-36γ, and IL-38 in individuals with healthy periodontium, gingivitis, and periodontitis and to evaluate their correlations with clinical periodontal parameters.
Ninety systemically healthy and nonsmoking volunteers divided into a healthy (H) group (n = 30), a gingivitis (G) group (n = 30), and a periodontitis (P) group (n = 30) were included in this study. Clinical periodontal parameters of volunteers were recorded, and GCF, unstimulated saliva, and serum samples were collected. Data analysis was done with enzyme-linked immunosorbent assays. The Kruskal-Wallis test and Bonferroni correction were used for multiple comparisons and post hoc statistical analyses.
The group H had significantly lower clinical parameters than the group P (p < 0.001). GCF and salivary IL-36γ and IL-38 levels were significantly higher in the group P than in the H and G groups (p < 0.05). Positive correlations between biochemical findings and clinical periodontal parameters were observed.
IL-36γ and IL-38 levels in GCF, saliva, and serum correlate with clinical periodontal parameters and may play a role in determining the activity of periodontitis.