目的:确定犬冻干血浆(cFDP)用无菌水连续稀释的特性。
方法:体外实验研究。
方法:政府血液和凝血研究实验室。
方法:cFDP来自商业制造商。
方法:将10个单位的cFDP重建为100%,90%,80%,70%,60%,50%,和40%的推荐体积与无菌水。分析所得溶液的凝血因子活性(因子II,V,VII,VIII,IX,X,和XII以及抗凝血酶),纤维蛋白原浓度,凝血酶原时间,活化部分凝血活酶时间,粘度,渗透压,和高岭土激活的血栓弹力图。
结果:粘度,渗透压,血浆的浊度特性以重建体积依赖性的方式增加,40%的建议体积产生约2倍的增加。同样,因子活性水平和纤维蛋白原浓度在此范围内以浓度依赖性方式增加约2倍。凝血酶原时间从100%体积时的11.4秒下降到70%时的10.9秒,然后增加到40%时的11.9秒。活化的部分凝血活酶时间从100%再水合时的21.8秒指数增加到40%时的100.0秒。在50%补液时,TEG的R时间从3.1增加到13.9分钟,而α角在相同范围内从61.3°下降到24.7°,并且最大振幅最初从100%水时的13.2mm增加到70%水时的18.6mm,然后下降回到50%水时的14.6mm。用40%的再水合没有观察到凝血。
结论:从cFDP产生高渗血浆似乎可行,保留浓缩的凝血因子,尽管仅使用推荐补液量的40%-50%测试的最高浓度时,凝血功能会发生一些无法解释的影响。需要进一步的研究来评估体内高渗产物。
OBJECTIVE: To determine the characteristics of canine freeze-dried plasma (cFDP) as it is serially diluted with sterile water.
METHODS: In vitro experimental study.
METHODS: Government blood and coagulation research laboratory.
METHODS: cFDP from a commercial manufacturer.
METHODS: Ten units of cFDP were reconstituted to 100%, 90%, 80%, 70%, 60%, 50%, and 40% of the recommended volume with sterile water. The resultant solutions were analyzed for coagulation factor activity (factors II, V, VII, VIII, IX, X, and XII as well as antithrombin), fibrinogen concentration, prothrombin time, activated partial thromboplastin time, viscosity, osmolality, and kaolin-activated thromboelastography.
RESULTS: Viscosity, osmolality, and turbidity properties of plasma were increased in a reconstitution volume-dependent manner, with the 40% suggested volume generating approximately 2-fold increases in each. Similarly, factor activity levels and fibrinogen concentration increased by approximately 2-fold over this range in a concentration-dependent manner. Prothrombin time declined from 11.4 seconds at 100% volume to 10.9 seconds at 70% before increasing to 11.9 seconds at 40%. Activated partial thromboplastin time increased exponentially from 21.8 seconds at 100% rehydration to 100.0 seconds at 40%. R-time on TEG increased from 3.1 to 13.9 minutes at 50% rehydration, while alpha angle declined from 61.3° to 24.7° over the same range, and the maximum amplitude initially increased from 13.2 mm at 100% water to 18.6 mm at 70% water before dropping back down to 14.6 mm at 50% water. No clotting was observed with 40% rehydration.
CONCLUSIONS: The creation of
hyperosmotic plasma from cFDP appears feasible with preservation of concentrated coagulation factors, although there are some unexplained effects that happen to coagulation functions at the highest concentrations tested using only 40%-50% of recommended rehydration volume. Further studies are needed to evaluate the
hyperosmotic product in vivo.