背景:5-羟色胺(5-HT)前体调节骨重建。本研究旨在探讨绝经后骨质疏松症(PMOP)患者血浆5-HT前体和代谢物与骨密度(BMD)和骨转换标志物的相关性。
方法:年龄,体重指数(BMI),并且记录了正常/骨质减少/骨质疏松症(OP)组中348名绝经后妇女的绝经年限(YSM),用腰椎和股骨颈测量骨密度。血清骨转换标志物(PINP/β-CTX)和血浆5-HT,通过ELISA测量5-HT前体(Trp/5-HTP)和代谢物(5-HIAA)。在5-HT/Trp/5-HTP/5-HIAA的ROC分析之后,将OP患者分配到高/低表达组。血浆5-HT/Trp/5-HTP/5-HIAA的关系,BMD,采用logistic回归分析PMOP骨转换标志物。血浆5-HT/Trp/5-HTP/5-HIAA与骨密度和骨转换标志物的相关性采用Pearson相关分析,然后对血浆5-HT/Trp/5-HTP/5-HIAA与骨密度的关系进行logistic回归分析,骨转换标记和PMOP。
结果:BMI,YSM,BMD和PINP,β-CTX水平在各组之间存在差异。OP患者血浆5-HT前体/代谢物水平升高。具有高5-HT前体/代谢物水平的个体具有低BMD和高PINP/β-CTX水平。5-HT前体/代谢物与BMD呈负相关,与PINP/β-CTX呈正相关。BMI,YSM,BMD,PINP/β-CTX/Trp/5-HTP/5-HT与PMOP相关,是OP的独立危险因素。
结论:PMOP患者血浆5-HT前体和代谢物与BMD呈负相关,与PINP/β-CTX呈正相关。外周5-HT前体和代谢产物水平可能是治疗PMOP和骨代谢相关疾病的新方向。
BACKGROUND: Serotonin (5-HT) precursors regulate bone remodeling. This study aims to investigate the correlation of plasma 5-HT precursors and metabolite with bone mineral density (BMD) and bone turnover markers in postmenopausal osteoporosis (PMOP) patients.
METHODS: The age, body mass index (BMI), and years since menopause (YSM) were documented for 348 postmenopausal women in normal/osteopenia/osteoporosis (OP) groups, with lumbar spine and femoral neck BMD measured. Serum bone turnover markers (PINP/β-CTX) and plasma 5-HT, 5-HT precursors (Trp/5-HTP) and metabolite (5-HIAA) were measured by ELISA. OP patients were allocated to high/low expression groups following ROC analysis of 5-HT/Trp/5-HTP/5-HIAA. The relationship of plasma 5-HT/Trp/5-HTP/5-HIAA, BMD, and bone turnover markers with PMOP was analyzed using logistic regression analysis. The correlation of plasma 5-HT/Trp/5-HTP/5-HIAA with BMD and bone turnover markers was analyzed using Pearson\'s correlation analysis, followed by logistic regression analysis of the relationship between plasma 5-HT/Trp/5-HTP/5-HIAA and BMD, bone turnover markers and PMOP.
RESULTS: BMI, YSM, BMD and PINP, and β-CTX levels differed among groups. Levels of plasma 5-HT precursors/metabolite were increased in OP patients. Individuals with high 5-HT precursors/metabolite levels had low BMD and high PINP/β-CTX levels. The 5-HT precursors/metabolite negatively-correlated with BMD and positively-correlated with PINP/β-CTX. BMI, YSM, BMD, and PINP/β-CTX/Trp/5-HTP/5-HT related to PMOP and were independent risk factors for OP.
CONCLUSIONS: Plasma 5-HT precursors and metabolite negatively-correlate with BMD and positively-correlate with PINP/β-CTX in PMOP patients. Peripheral 5-HT precursors and metabolite level may be a new direction of treatment of PMOP and bone metabolism-related disorders.