埃克托因和羟基埃克托因是在医疗和化妆品工业中使用的具有巨大潜力的相容溶质。考虑到这些相容溶质的优异渗透保护特性,我们调查了四种相容溶质的存在(Ettoine,羟基艾托宁,脯氨酸,和谷氨酸)通过液相色谱-串联质谱(LC-MS/MS)对45种嗜盐/耐盐细菌分离株进行定量。我们确定了Marinibacillussp。,Nesterenkonia信江,Halobacillussp.,巴塔哥尼芽孢杆菌,picturae,蛇形盐单胞菌,巴塔哥尼芽孢杆菌,芽孢杆菌。,亚拉西杆菌,微细菌。,Nesterenkoniasp.,和芽孢杆菌,这种生产依赖于NaCl。此外,在6种细菌分离物中观察到羟基艾托宁的产生(NesterenkoniaXinjiangensis,Halobacillussp.,蛇形盐单胞菌,亚拉西杆菌,Nesterenkoniasp.,和芽孢杆菌Agaradhaerens),与NaCl和温度有关。该研究确定了产生艾克托因或羟基艾克托因的新细菌分离株。虽然许多不同的芽孢杆菌属成员和一些Nesterenkonia中的外胎生产已经被记录,到目前为止,尚未显示巴塔哥尼芽孢杆菌和新疆Nesterenkonia的外齿生产。Further,首次通过实验证明了Thalassobacillus属(Thalassobacillusdevorans)的成员生产的外胎。该研究报告的发现可能为将来大规模生产ectoine和羟基ectoine奠定基础。
Ectoine and
hydroxyectoine are compatible solutes with enormous potential for use in the medical and cosmetic industries. Considering the excellent osmoprotective properties of these compatible solutes, we investigate the presence of four compatible solutes (ectoine,
hydroxyectoine, proline, and glutamic acid) quantitatively by liquid chromatography-tandem mass spectrometry (LC-MS/MS) in forty-five halophilic/halotolerant bacterial isolates. We determined ectoine production by Marinibacillus sp., Nesterenkonia xinjiangensis, Halobacillus sp., Bacillus patagoniensis, Virgibacillus picturae, Halomonas neptunia, Bacillus patagoniensis, Gracilibacillus sp., Thalassobacillus devorans, Microbacterium sp., Nesterenkonia sp., and Bacillus agaradhaerens, and this production was NaCl dependent. Additionally, the production of
hydroxyectoine was observed in six bacterial isolates (Nesterenkonia xinjiangensis, Halobacillus sp., Halomonas neptunia, Thalassobacillus devorans, Nesterenkonia sp., and Bacillus agaradhaerens) which was NaCl and temperature dependent. The study identified new bacterial isolates producing ectoine or
hydroxyectoine. While the ectoine production in many different Bacillus members and a few Nesterenkonia have been documented before, ectoine production by Bacillus patagoniensis and Nesterenkonia xinjiangensis has not been shown so far. Further, ectoine production by a member of the genus Thalassobacillus (Thalassobacillus devorans) was demonstrated experimentally for the first time. The findings reported in the study may serve as a basis for the large-scale production of ectoine and
hydroxyectoine in the future.