红曲色素(RMP),一种天然色素,由于其适合食品使用和潜在的健康益处而引起了极大的关注。然而,维护其稳定性和探索增值发展机遇仍然是关键挑战。这项研究概述了RMP的利用,通过Ca2+介导的壳聚糖(CS)/海藻酸钠(SA)包封(CO-RMPHB)成功制备包封RMP粗提物(RMPCE)的水凝胶珠。对制造和稳定性参数进行了系统的研究,包括准备条件,温度,单色光和存储时间,进行了。通过优化(SA:2.50wt%;CaCl2:6.00wt%;CS:0.50wt%),达到73.54±2.16%的最大包封效率。空白水凝胶珠(BHB)在模拟胃液(pH=1.2,1.50±0.97%)中的最大溶胀度明显低于模拟肠液(pH=7.0,28.05±1.43%),确认它们对pH变化的敏感性。此外,CO-RMPHB(66.08%,与单个RMPCE或BHB相比,1000μL)表现出优异的DPPH自由基清除能力。此外,基于零级的释放动力学分析,一阶,Higuchi,和Ritger-Peppas模型表明,在体外消化模型下,RMPCE从CO-RMPHB释放遵循非Fickian扩散。这一发现有效地解决了RMP的稳定性和控释的挑战。扩大其在食品和制药行业的应用。
Red Monascus Pigment (RMP), a natural pigment, has attracted significant attention due to its suitability for food use and potential health benefits. However, preserving its stability and exploring value-added development opportunities remain crucial challenges. This study outlined the utilization of RMP, by successfully preparing hydrogel beads encapsulating RMP crude extract (RMPCE) through Ca2+-mediated chitosan (CS)/sodium alginate (SA) encapsulation (CO-RMPHB). A systematic investigation into the fabrication and stability parameters, including preparation conditions, temperature, monochromatic light and storage time, was undertaken. Through optimization (SA: 2.50 wt%; CaCl2: 6.00 wt%; CS: 0.50 wt%), maximum encapsulation efficiency of 73.54 ± 2.16 % was achieved. The maximum swelling degree of blank hydrogel beads (BHB) in simulated gastric solution (pH = 1.2, 1.50 ± 0.97 %) was significantly lower than in simulated intestinal solution (pH = 7.0, 28.05 ± 1.43 %), confirming their sensitivity to pH changes. Additionally, the CO-RMPHB (66.08 %, 1000 μL) exhibited superior DPPH radical scavenging capability compared to individual RMPCE or BHB. Furthermore, analysis of the release kinetics based on zero-order, first-order, Higuchi, and Ritger-Peppas models revealed that RMPCE release from CO-RMPHB under in vitro digestion models followed non-Fickian diffusion. This discovery effectively addresses the challenges of the stability and controlled release of RMP, expanding its applications in the food and pharmaceutical industries.