溶菌酶通常用作饲料添加剂,作为抗菌蛋白,增强牲畜和家禽的免疫系统,同时防止病原体。研究毕赤酵母重组人溶菌酶(rhLYZ)和金霉素对肉鸡生产性能的影响,抗氧化特性,和肠道微生物群,总共选择200只一天大的雄性ArborAcres肉鸡(46.53±0.42g)进行42天的实验。饮食处理包括:补充0mg/kg(CON)的玉米豆粕的基础饮食,50mg/kg金霉素(ANT),20mg/kgrhLYZ(低),60mg/kgrhLYZ(中),或180毫克/千克rhLYZ(高)。与CON相比,从第22天到第42天,中等饮食增加了(p<0.05)肉鸡的平均日增重(ADG)(=67.40g)。在早期(=1.29)和整体阶段(=1.69),中等导致肉鸡的饲料转化率(FCR)降低(P<0.05)。此外,与CON和ANT相比,培养基显示血清中INF-γ和TNF-α水平降低(P<0.05)。在盲肠,与CON相比,在中等治疗中,Monoglobus和Family_XIII_AD3011_组的丰度较低(P<0.05)。总的来说,补充60mg/kg的rhLYZ提高了生长性能,养分利用效率,和血清免疫功能,同时也影响肠道微生物群的组成。这表明溶菌酶有可能替代饲料中的抗生素添加剂。
Lysozyme is often used as a feed additive to act as an antibacterial protein that boosts the immune system of livestock and poultry while protecting against pathogens. To investigate the effects of recombinant human lysozyme (rhLYZ) from Pichia pastoris and chlortetracycline on broiler chicken\'s production performance, antioxidant characteristics, and intestinal microbiota, a total of 200, 1-d-old male Arbor Acres broiler chickens (46.53 ± 0.42 g) were selected for a 42-d experiment. Dietary treatments included a basal diet of corn-soybean meal supplemented with either 0 mg/kg (CON), 50 mg/kg aureomycin (ANT), 20 mg/kg rhLYZ (LOW), 60 mg/kg rhLYZ (MEDIUM), or 180 mg/kg rhLYZ (HIGH). Compared with CON, MEDIUM diet increased (P < 0.05) average daily gain (67.40 g) of broilers from day 22 to 42. In the early (1.29) and overall phases (1.69), MEDIUM led to a reduction (P < 0.05) in the feed conversion ratio of broiler chickens. Furthermore, in comparison to the CON and ANT, MEDIUM exhibited reduced (P < 0.05) levels of INF-γ and tumor necrosis factor-α in the serum. In the cecum, the abundance of Monoglobus and Family_XIII_AD3011_group was lower (P < 0.05) in the MEDIUM treatment compared to CON. Overall, supplementation of 60 mg/kg of rhLYZ improved growth performance, nutrient utilization efficiency, and serum immune function, while also influencing the composition of intestinal microbiota. This suggests lysozyme\'s potential to replace antibiotic additives in feed.
The aim of this study was to explore the effects of recombinant human lysozyme (rhLYZ) produced from Pichia pastoris and chlortetracycline on broiler chicken performance, antioxidant properties, and gut microbiota. A 42-d experiment was conducted, involving 200 1-d-old male Arbor Acres broiler chickens. We provided different diets: a standard diet (CON), a diet with 50 mg/kg aureomycin (ANT), a diet with 20 mg/kg rhLYZ (LOW), a diet with 60 mg/kg rhLYZ (MEDIUM), or a diet with 180 mg/kg rhLYZ (HIGH). The results showed that, compared to the control group, the MEDIUM group significantly increased the average daily gain of broilers to 67.40 g from day 22 to 42. Additionally, the MEDIUM group exhibited a reduced feed conversion ratio during both the early and overall growth stages of the chickens. Furthermore, serum levels of INF-γ and tumor necrosis factor-α were lower in the MEDIUM group compared to both the CON and ANT groups. In the cecum, the abundance of Monoglobus and Family_XIII_AD3011_group was also lower in the MEDIUM treatment compared to the CON group. Overall, supplementation with 60 mg/kg of rhLYZ improved growth performance, nutrient utilization efficiency, and serum immune function in broiler chickens while also influencing the composition of their intestinal microbiota. This suggests the potential of lysozyme as a replacement for antibiotic additives in feed.