传统光学显微镜的空间分辨率受衍射极限限制为数百纳米。超分辨率显微镜通过规避传统光学显微镜的衍射极限，实现一位数纳米分辨率。用于纳米级形貌成像的DNA点积累（DNA-PAINT）属于单分子定位超分辨率方法家族。DNA-PAINT的独特功能是它允许亚纳米分辨率，频谱无限复用，接近检测,和目标分子的定量计数。这里,我们描述了有效的DNA-PAINT显微镜检查的先决条件。
The spatial resolution of conventional light microscopy is restricted by the diffraction limit to hundreds of nanometers. Super-resolution microscopy enables single digit nanometer resolution by circumventing the diffraction limit of conventional light microscopy. DNA point accumulation for imaging in nanoscale topography (DNA-PAINT) belongs to the family of single-molecule localization super-resolution approaches. Unique features of DNA-PAINT are that it allows for sub-nanometer resolution, spectrally unlimited multiplexing, proximity detection, and quantitative counting of target molecules. Here, we describe prerequisites for efficient DNA-PAINT microscopy.
