■由坏死真菌Ascochytarabiei引起的Ascochyta疫病(AB)是限制鹰嘴豆生产的最重要的疾病之一。了解鹰嘴豆-A的代谢机制。rabiei相互作用将为开发新的方法来管理这种疾病提供重要的线索。
■我们对两个鹰嘴豆种质的气生组织(叶和茎)进行了代谢物分析,这些鹰嘴豆种质包括中度抗性育种系(CICA1841)和高度易感品种(Kyabra),以响应一种使用液相色谱-质谱的非靶向代谢组学分析澳大利亚A.rabiei分离株TR9571。
■结果显示抗性和易感性相关的组成型代谢物,例如中等抗性育种系的阿魏酸质量丰度较高,而儿茶素水平较高,邻苯二甲酸,烟酸在易感品种中含量较高。Further,宿主-病原体相互作用导致各种代谢物水平的改变(诱导和抑制),特别是在易感品种中,揭示了对A.rabiei敏感的可能原因。值得注意的是,真菌定植后,在易感品种的气生组织中诱导了大量的水杨酸,茉莉酸甲酯(MeJA)被抑制,阐明植物激素在鹰嘴豆A中的关键作用拉比互动。类黄酮生物合成中的许多差异代谢物,苯丙氨酸,氨酰tRNA生物合成,戊糖和葡糖醛酸酯相互转化,精氨酸生物合成,缬氨酸,亮氨酸,和异亮氨酸的生物合成,还有丙氨酸,天冬氨酸,谷氨酸代谢途径上调和下调,表明这些代谢途径参与鹰嘴豆A。拉比互动。
■放在一起,这项研究强调了鹰嘴豆-A.rabiei在代谢物水平上的相互作用,并显示了A.rabiei如何差异地改变中度抗性和易感鹰嘴豆的代谢物特征,并且可能正在利用鹰嘴豆的防御途径。
UNASSIGNED: Ascochyta blight (AB) caused by the necrotrophic fungus Ascochyta rabiei is one of the most significant diseases that limit the production of chickpea. Understanding the metabolic mechanisms underlying chickpea-A.rabiei interactions will provide important clues to develop novel approaches to manage this disease.
UNASSIGNED: We performed metabolite profiling of the aerial tissue (leaf and stem) of two chickpea accessions comprising a moderately resistant breeding line (CICA1841) and a highly susceptible cultivar (Kyabra) in response to one of the highly aggressive Australian A. rabiei isolates TR9571 via non-targeted metabolomics analysis using liquid chromatography-mass spectrometry.
UNASSIGNED: The results revealed resistance and susceptibility-associated constitutive metabolites for example the moderately resistant breeding line had a higher mass abundance of ferulic acid while the levels of catechins, phthalic acid, and nicotinic acid were high in the susceptible cultivar. Further, the host-pathogen interaction resulted in the altered levels of various metabolites (induced and suppressed), especially in the susceptible cultivar revealing a possible reason for susceptibility against A.r abiei. Noticeably, the mass abundance of salicylic acid was induced in the aerial tissue of the susceptible cultivar after fungus colonization, while methyl jasmonate (MeJA) was suppressed, elucidating the key role of phytohormones in chickpea-A. rabiei interaction. Many differential metabolites in flavonoid biosynthesis, phenylalanine, Aminoacyl-tRNA biosynthesis, pentose and glucuronate interconversions, arginine biosynthesis, valine, leucine, and isoleucine biosynthesis, and alanine, aspartate, and glutamate metabolism pathways were up- and down-regulated showing the involvement of these metabolic pathways in chickpea-A. rabiei interaction.
UNASSIGNED: Taken together, this study highlights the chickpea - A. rabiei interaction at a metabolite level and shows how A. rabiei differentially alters the metabolite profile of moderately resistant and susceptible chickpea accessions and is probably exploiting the chickpea defense pathways in its favour.