Providencia物种是人类重要的机会病原体,并与几种传染病有关。在这项研究中,我们发现了三种属于一种新的普罗维登西亚物种的临床菌株,也就是华山普罗维登西亚,包括菌株CRE-3FA-0001T,CRE-138-0026,andCRE-138-0111.这些菌株是从三名患者身上回收的,所有这些都与医院感染有关,包括手术部位,泌尿道,颅内感染.这三个菌株对许多类型的抗微生物剂显示出高水平的抗性,包括阿米卡星,氨曲南,头孢他啶,头孢吡肟,环丙沙星,粘菌素,多粘菌素B,亚胺培南,美罗培南,头孢他啶-阿维巴坦,亚胺培南-莱巴坦.抗性机制的研究表明,获得性抗性基因如blaKPC,blaNDM,BlaPER,blaOXA,aac,蚂蚁,qnrD,等。,在3株多重耐药表型中发挥了重要作用。根据16SrRNA基因序列重建系统发育树,多位点序列分析,和核心SNP。菌株的基因组序列与其他Providencia型菌株的平均核苷酸同一性(ANI)为83.5-85.8%,计算机DNA-DNA杂交(isDDH)评分为21-25.5%。ANI和isDDH值和系统发育树表明,菌株CRE-3FA-0001T,CRE-138-0026和CRE-138-0111菌株应被视为普罗维登西亚属的新物种,名字为P.huashanensissp.11月。是提议的。菌株类型为CRE-3FA-0001T=CCTCCAB2023186T=KCTC8373T。
Providencia species are important opportunistic pathogens for humans and are associated with several infectious diseases. In this study, we found three clinical strains belonging to a novel Providencia species, namely Providencia huashanensis, including strains CRE-3FA-0001T, CRE-138-0026, and CRE-138-0111. These strains were recovered from three patients, and all of them were associated with nosocomial infections, including incision infection, urinary tract infection, and intracranial infection. The three strains showed high-level resistance to many types of antimicrobials, including amikacin, aztreonam, ceftazidime, cefepime, ciprofloxacin, colistin, polymyxin B, imipenem, meropenem, ceftazidime-avibactam, imipenem-relebactam. Investigation of the resistance mechanism revealed that acquired resistance genes such as blaKPC, blaNDM, blaPER, blaOXA, aac, ant, and qnrD, played an important role in the multidrug-resistant phenotype for the three strains. The phylogenetic trees were reconstructed based on the 16S rRNA gene sequences, multi-locus sequence analysis, and core single nucleotide polymorphisms. The genome sequence of the strains had a range of 83.5%-85.8% average nucleotide identity and 21%-25.5% in silico DNA-DNA hybridization scores with other Providencia type strains. The average nucleotide identity and in silico DNA-DNA hybridization values and the phylogenetic trees indicated that the strains CRE-3FA-0001T, CRE-138-0026, and CRE-138-0111 strains should be considered as a novel species of the genus Providencia, for which the name P. huashanensis sp. nov. is proposed. The type strain is CRE-3FA-0001T = China Center for Type Culture Collection AB 2023186T = Korean Collection for Type Cultures 8373T.