背景:尚无关于在小鼠中成功实施星状神经节阻滞(SGB)的报道。
目的:本研究旨在研究一种在小鼠中实施SGB的新方法,方法是将其置于仰卧位并外展上肢并用手触摸气管和胸锁关节。
方法:50只BABL/C小鼠,8-10周,随机分为4组:对照组(n=5);SGB-R组(n=15);SGB-L组(n=15);SGB-L+R组(n=15)。SGB与0.ImL体积的0.15%罗哌卡因溶液一起施用。对照组接受等体积的盐水。霍纳综合征,心率,以及臂丛神经阻滞等并发症,血管损伤,气胸,局部麻醉毒性,并观察到死亡。
结果:霍纳综合征在100%的SGB存活小鼠中发展;发病时间没有差异(100.4±13.4vs96.7±12.4,平均值±SD,秒)和持续时间(264.1±40.5vs296.3±48.0,平均值±SD,左侧和右侧SGB的霍纳综合征的最小值)(P>0.05)。与对照组相比(722[708-726],中位数[IQR],bpm),右SGB的心率显着减慢(475[451.5-491],中位数[IQR],bpm)(P<0.05)。当执行左SGB后心率减慢时,差异无统计学意义(P>0.05)。总并发症发生率为18.4%,臂丛神经阻滞率为12.3%,血管损伤率为4.6%,死亡率为1.5%,以及未发现局部麻醉毒性(包括双侧实施SGB)或气胸表现。
结论:该方法允许在小鼠模型中成功实施SGB。
BACKGROUND: There have been no reports on the successful implementation of stellate ganglion block (SGB) in mice.
OBJECTIVE: This study aims to investigate a new method for implementing SGB in mice by placing them in a supine position with abducted upper limbs and touching the trachea and sternoclavicular joint with the hand.
METHODS: Fifty BABL/C mice, 8-10 weeks, were selected and randomly divided into four groups: control group (n = 5); SGB-R group (n = 15); SGB-L group (n = 15); and SGB-L + R (group n = 15). SGB was administered with 0.15% ropivacaine solution in a volume of 0.1 mL. The control group received equal volumes of saline. Horner\'s syndrome, heart rate, and complications such as brachial plexus block, vascular injury, pneumothorax, local anesthetic toxicity, and death were observed.
RESULTS: Horner\'s syndrome developed in 100% of SGB surviving mice; no difference was seen in the time to onset (100.4 ± 13.4 vs 96.7 ± 12.4, mean ± SD, seconds) and duration (264.1 ± 40.5 vs 296.3 ± 48.0, mean ± SD, min) of Horner\'s syndrome in the left and right SGB (P > 0.05). Compared with the control group (722 [708-726], median [IQR], bpm), the heart rate was significantly slowed down in the right SGB (475 [451.5-491], median [IQR], bpm) (P < 0.05). While the heart rate was slowed down after performing the left SGB, the difference was not statistically significant (P > 0.05). The overall complication rate was 18.4%, with a brachial plexus block rate of 12.3%, a vascular injury rate of 4.6%, and a mortality rate of 1.5%, as well as no local anesthetic toxicity (includes bilateral implementation of SGB) or pneumothorax manifestations were found.
CONCLUSIONS: This method allows for the successful implementation of SGB in a mouse model.