meleagridis,原生动物寄生虫,在家禽中诱发黑头病(组织烟虫病)。在孵化过程中,对绵羊红细胞的高(HAS)和低(LAS)抗体反应进行不同选择的小鸡分为两组,每个HAS和LAS,并放在有木屑的钢笔里作为垃圾。随意允许进料和水。来自每个品系的一半雏鸡都有罗伊利氏杆菌(L.reuteri)接种到他们的饮用水中。在第18天,对所有雏鸡进行了100,000个H.meleagridis细胞的经cloacal接种。然后,10天后,他们被安乐死,然后从大脑中收集组织,盲肠扁桃体,Ceca,肝脏,胸腺,和脾脏用于参与免疫学发育的细胞因子的qPCR分析。细胞因子表达的变化在盲肠扁桃体中最多,Ceca,还有肝脏.在没有用于控制组织滴虫病的功能性药物的情况下,罗伊氏乳杆菌和/或其分泌产物,reuterin,可能有用,在一些遗传群体中,作为一种手段,以减少对鸡的影响。数据表明,罗伊氏乳杆菌治疗在两个遗传系之间具有组织特异性,其中作用主要针对盲肠扁桃体,Ceca,还有肝脏,是寄生虫的主要组织靶标(H.meleagridis),还有胸腺和脾脏.然而,主要效应之间的相互作用反映了在一个基因系的组织中观察到的对炎症标志物的反应可能在另一个基因系中未观察到。
Histomonas meleagridis, a protozoan parasite, induces blackhead disease (histomoniasis) in poultry. During hatching, chicks from lines divergently selected for high (HAS) and low (LAS) antibody responses to sheep red blood cells were divided into two groups, each of HAS and LAS, and placed in pens with wood shavings as litter. Feed and water were allowed ad libitum. Half of the chicks from each line had Limosilactobacillus reuteri (L. reuteri) inoculated to their drinking water. On day 18, all chicks were given a transcloacal inoculation of 100,000 H. meleagridis cells. Then, 10 days later, they were euthanized, followed by collection of tissues from the brain, cecal tonsil, ceca, liver, thymus, and spleen for qPCR analyses of cytokines involved in immunological development. Changes in cytokine expressions were most numerous in the cecal tonsil, ceca, and liver. In the absence of a functional medication for control of histomoniasis, L. reuteri and/or its secretory product, reuterin, might serve, in some genetic populations, as a means to reduce the impact of histomoniasis in chickens. The data demonstrate that L. reuteri treatment had tissue specificity between the two genetic lines, in which the effects were targeted primarily toward the cecal tonsil, ceca, and liver, which are the primary tissue targets of the parasite (H. meleagridis), as well as the thymus and spleen. However, interactions among main effects reflect that responses to inflammatory markers observed in tissues for one genetic line may not be observed in another.