现有证据表明西咪替丁是一种生殖毒物,可引起性和睾丸功能障碍。Gratissimum(OG)在全球范围内用于医疗和营养目的。为了确定西咪替丁对西咪替丁引起的性腺毒性的调节作用,将性成熟的雄性大鼠随机分为4组,每组6只(n=6).A组:对照组给予2ml蒸馏水。B组接受500mg/kg体重(bwt)的OG提取物,C组接受50mg/kgbwt西咪替丁,D组通过灌胃每天一次接受50mg/kgbwt的西咪替丁+500mg/kgbwtOG提取物,共8周。测试的参数包括精子参数,睾酮(TT),黄体生成素(LH),促卵泡激素(FSH)和催乳素,睾丸碱性磷酸酶(ALP),酸性磷酸酶(ACP),乳酸脱氢酶(LDH),蛋白质,胆固醇,糖原,性行为参数,还有睾丸组织学.
生精上皮有精减,精子质量下降,TT,LH,FSH,睾丸酶,蛋白质,胆固醇,糖原,与对照组相比,仅用西咪替丁治疗的动物的性行为增加。OG恢复并改善了性行为和性欲,从增加的坐骑频率可以明显看出,intromission,射精,和射精潜伏期。挂载延迟,intromission,射精后,催乳素明显下降。ALP的睾丸活性明显降低,ACP,LDH和蛋白质,胆固醇,糖原浓度,TT,OG给药使LH和FSH增加。
西咪替丁对睾丸的有害作用减弱,保护了生精上皮,恢复,提高了性能力,促进精子发生。
Available evidence suggests that cimetidine is a reproductive toxicant that induces sexual and testicular dysfunction. Ocimum gratissimum (OG) is globally consumed for medicinal and nutritional purposes. To determine the modulating role of aqueous leaf extract of Ocimum gratissimum on cimetidine-induced gonado-toxicity, sexually mature male rats were randomized into four groups of six (n=6) rats each. Group A: control given 2ml distilled water. Group B received 500mg/kg body weight (bwt) of OG extract, Group C received 50mg/kg bwt cimetidine, and group D received 50mg/kg bwt of cimetidine+500mg/kg bwt OG extract once daily for 8 weeks via gastric gavage. Parameters tested include sperm parameters, testosterone (TT), luteinizing hormone (LH), follicle stimulating hormone (FSH) and prolactin, testicular alkaline phosphatase (ALP), acid phosphatase (ACP), lactate dehydrogenase (LDH), protein, cholesterol, glycogen, sexual behavioural parameters, and testicular histology.
There were depletions in the seminiferous epithelium, decreased sperm quality, TT, LH, and FSH, testicular enzymes, protein, cholesterol, glycogen, and sexual behaviour increase in animals treated with cimetidine only compared to control. OG restored and improved sexual behaviour and libido as evident from increased frequencies of mount, intromission, ejaculation, and ejaculatory latency. Mount latencies, intromission, post-ejaculation, and prolactin were significantly decreased. The significantly decreased testicular activities of ALP, ACP, LDH and protein, cholesterol, glycogen concentrations, TT, LH and FSH were increased by OG administration.
Ocimum gratissimum attenuated the deleterious effects of cimetidine on the testis, protected the seminiferous epithelium, restored, and boosted sexual competence, and promoted spermatogenesis.