目的:翻译控制肿瘤蛋白(TCTP)是大多数真核生物中功能重要的蛋白,因为它参与了多种过程,最具代表性的是扩散,分化,组胺释放,细胞死亡,蛋白质合成和对胁迫条件的反应。在目前的工作中,我们分析序列,一组人类寄生原生动物中TCTP直系同源物的结构和系统发育。
方法:使用EuPathDB生物信息学资源中心数据库中的NCBIBLAST工具鉴定了原生动物寄生虫中TCTP直向同源物的完整序列。对序列进行比对,并鉴定了蛋白质的重要区域,后来用不同的生物信息学工具建立了系统发育树和3D模型。
结果:我们的结果显示了进化上和结构上保守的位点,考虑到对当前药物耐药的菌株数量的增加,这些位点可用于创造新的治疗策略。
结论:对原生动物寄生虫中的TCTP直系同源物的研究很少,但已被证明在寄生虫生长中很重要,扩散,繁殖,以及对环境变化的反应。对于这一切,TCTP可以被认为是可能的治疗靶标。
OBJECTIVE: Translational controlled tumor protein (TCTP) is a functionally important protein in most eukaryotes because it participates in a wide variety of processes, the most representative being proliferation, differentiation, histamine release, cell death, protein synthesis and response to stress conditions. In the present work, we analyze the sequence, structure and phylogeny of TCTP orthologs in a group of human parasitic protozoan species.
METHODS: The complete sequences of TCTP orthologs in protozoan parasites were identified with the NCBI BLAST tool in the database of the EuPathDB Bioinformatics Resource Center. The sequences were aligned and important regions of the protein were identified, and later phylogenetic trees and 3D models were built with different bioinformatic tools.
RESULTS: Our results show evolutionarily and structurally conserved sites that could be exploited to create new therapeutic strategies given the increase in the number of strains resistant to current drugs.
CONCLUSIONS: TCTP orthologs in protozoan parasites have been little studied but have been shown to be important in parasite growth, proliferation, reproduction, and response to changes in the environment. For all this, TCTP can be considered as a possible therapeutic target.