即使在同一个房间里发酵,大曲将形成各种微生态。在不同的层中观察到微生物种群生态位的逐渐分化,表现为优势微生物包括芽孢杆菌的丰度变化,糖聚孢子菌,Weissella,Kroppenstedtia,热曲,热细菌,酵母菌,还有Rasamsonia.此外,在微生物的功能表达中观察到差异,包括曲霉,Virgibacillus,海洋芽孢杆菌,和神经孢菌.中层大曲群落表现出高密实度和生态位多样性的特征,这促进了有效的底物利用。在初始阶段,上大曲社区表现出活动增强。然而,在中层和下层,真菌和细菌分别表现出更大的功能表达。调节上层和中层群落组装的关键环境因素是pH和温度,分别,较低的是水分和酸度。值得注意的是,确定性装配对真菌的影响更大。
Even if fermented in the same qu-room, Daqu will form various microecologies. A gradual differentiation of microbial population niches was observed within different qu-layers, manifesting as variations in the abundance of dominant microorganisms including Bacillus, Saccharopolyspora, Weissella, Kroppenstedtia, Thermoascus, Thermomyces, Saccharomycopsis, and Rasamsonia. Moreover, distinctions were observed in the functional expression of microorganisms, including Aspergillus, Virgibacillus, Oceanobacillus, and Neurospora. The community in middle layer Daqu exhibited characteristics of high compactness and niche diversity, which facilitated efficient substrate utilization. During the initial phase, the upper Daqu community demonstrated heightened activity. However, in the middle and lower layers, fungi and bacteria respectively exhibited greater functional expression. The key environmental factors regulating the assembly of communities in the upper and middle layers were pH and temperature, respectively, and the lower was moisture and acidity. Notably, deterministic assembly exerted a stronger influence on fungi.