在大豆育种系11-452(高度抗性)和Dongsheng1(DS1,高度易感)中解开大豆囊肿线虫(Heteroderaglycines)种族4抗性和敏感性的复杂性是这项研究的重点。采用尖端的N6-甲基腺苷(m6A)和RNA测序技术,我们探讨了m6A修饰对基因表达和植物防御反应的影响。通过对抗性和易感根中线虫发育的评估,确定了m6A甲基化测序的关键时间点(接种后3天).我们的测序数据表现出稳健的统计数据,成功的大豆基因组作图,和普遍的M6A峰分布,主要在3'非翻译区和终止密码子区。差异甲基化峰和差异表达基因的分析揭示了抗性和易感基因型之间的独特模式。在高抗性生产线(11-452)中,关键抗性和防御相关基因显示表达增加,甲基化抑制,包括R基因等关键角色,受体激酶,和转录因子。相反,高度易感的DS1品系在与易感途径相关的基因中表现出与甲基化降低相关的高表达,包括霉菌基因座O样蛋白和影响防御机制的调节元件。全基因组评估,基因本体论和京都百科全书的基因和基因组分析,差异甲基化峰/差异表达基因重叠强调了m6A修饰的复杂相互作用,交替拼接,microRNA,和植物防御中的基因调控。蛋白质-蛋白质相互作用网络照亮防御关键基因,描述抗性和易感反应中的不同机制。本研究揭示了甲基化之间的动态相关性,拼接,和基因表达,为植物对线虫感染的反应提供深刻的见解。
Unraveling the intricacies of soybean cyst nematode (Heterodera glycines) race 4 resistance and susceptibility in soybean breeding lines-11-452 (highly resistant) and Dongsheng1 (DS1, highly susceptible)-was the focal point of this study. Employing cutting-edge N6-methyladenosine (m6A) and RNA sequencing techniques, we delved into the impact of m6A modification on gene expression and plant defense responses. Through the evaluation of nematode development in both resistant and susceptible roots, a pivotal time point (3 days postinoculation) for m6A methylation sequencing was identified. Our sequencing data exhibited robust statistics, successful soybean genome mapping, and prevalent m6A peak distributions, primarily in the 3\' untranslated region and stop codon regions. Analysis of differential methylation peaks and differentially expressed genes revealed distinctive patterns between resistant and susceptible genotypes. In the highly resistant line (11-452), key resistance and defense-associated genes displayed increased expression coupled with inhibited methylation, encompassing crucial players such as R genes, receptor kinases, and transcription factors. Conversely, the highly susceptible DS1 line exhibited heightened expression correlated with decreased methylation in genes linked to susceptibility pathways, including Mildew Locus O-like proteins and regulatory elements affecting defense mechanisms. Genome-wide assessments, Gene Ontology and Kyoto Encyclopedia of Genes and Genomes analyses, and differential methylation peak/differentially expressed gene overlap emphasized the intricate interplay of m6A modifications, alternative splicing, microRNA, and gene regulation in plant defense. Protein-protein interaction networks illuminated defense-pivotal genes, delineating divergent mechanisms in resistant and susceptible responses. This study sheds light on the dynamic correlation between methylation, splicing, and gene expression, providing profound insights into plant responses to nematode infection.