■各种研究表明,肠道菌群与自身免疫性疾病之间存在联系,但与过敏性紫癜(HSP)和免疫性血小板减少性紫癜(ITP)的因果关系尚不清楚.
■这项研究使用孟德尔随机化(MR)调查了肠道微生物群与HSP和ITP之间的双向因果关系。利用了来自MiBioGen财团和荷兰微生物组项目的门至物种水平的肠道微生物群的大规模遗传数据。HSP和ITP的全基因组关联研究(GWAS)汇总统计来自FinnGenR10。各种MR方法被用来推断因果关系,包括逆方差加权(IVW),最大似然(ML),CML-MA,MR-Egger,加权中位数,加权模型,MR-PRESSO进行了多重敏感性分析和Bonferroni校正以增强鲁棒性和可靠性。
■根据IVW估计,鉴定了23个细菌分类群与HSP和ITP具有暗示性关联。值得注意的是,Bonferroni校正后,alcaligenaceae(OR=2.86,95%CI=1.52-5.37;IVW,p=1.10×10-3,ML,p=1.40×10-3)与ITP作为危险因素显着相关,而家族拟杆菌S247组(OR=0.46,95%CI=0.29-0.74;IVW,p=1.40×10-3)与ITP作为保护因素显着相关。在反向分析中没有发现HSP和ITP与肠道微生物群之间的显著关联。
■我们的研究提供了肠道菌群对HSP和ITP的因果影响的证据,强调进一步研究以阐明这些自身免疫性疾病的潜在机制并开发有针对性的治疗干预措施的重要性。
UNASSIGNED: A variety of studies have shown a link between the gut microbiota and autoimmune diseases, but the causal relationship with Henoch-Schönlein purpura (HSP) and immune thrombocytopenic purpura (ITP) is unknown.
UNASSIGNED: This study investigated the bidirectional causality between gut microbiota and HSP and ITP using Mendelian randomization (MR). Large-scale genetic data of gut microbiota at phylum to species level from the MiBioGen consortium and the Dutch Microbiome Project were utilized. Genome-wide association studies (GWAS) summary statistics for HSP and ITP came from FinnGen R10. Various MR methods were applied to infer causal relationships, including inverse variance weighted (IVW), maximum likelihood (ML), cML-MA, MR-Egger, weighted median, weighted model, and MR-PRESSO. Multiple sensitivity analyses and Bonferroni correction were conducted to enhance robustness and reliability.
UNASSIGNED: Based on the IVW estimates, 23 bacterial taxa were identified to have suggestive associations with HSP and ITP. Remarkably, after Bonferroni correction, family Alcaligenaceae (OR = 2.86, 95% CI = 1.52-5.37; IVW, p = 1.10 × 10-3, ML, p = 1.40 × 10-3) was significantly associated with ITP as a risk factor, while family Bacteroidales S24 7group (OR = 0.46, 95% CI = 0.29-0.74; IVW, p = 1.40 × 10-3) was significantly associated with ITP as a protective factor. No significant associations between HSP and ITP and gut microbiota were found in reverse analyses.
UNASSIGNED: Our study provides evidence of causal effects of gut microbiota on HSP and ITP, highlighting the importance of further research to clarify the underlying mechanisms and develop targeted therapeutic interventions for these autoimmune diseases.