目的:微生物细胞耐受各种抗菌药物作用的能力代表了全球主要的健康问题。柔性和多组分纳米复合材料具有增强的物理化学特性和一些改进的性能。因此,评估了生物合成淀粉/银硒纳米复合材料(St/Ag-SeNC)的不同生物活性。
方法:使用CladosporiumCBS174.62(C.cladosporioides)菌株。使用扫描电子显微镜(SEM)和高分辨率透射电子显微镜(HR-TEM)研究了形状和平均粒径,分别。另一方面,评估了St/Ag-SeNC对两种癌细胞系和红细胞(RBC)的作用,并评估了其过氧化氢(H2O2)清除作用。此外,检查了其对浮游生物和生物膜生长形式的各种微生物物种的影响。
结果:成功地生物合成了St/Ag-SeNC,其具有椭圆形和球形形状以及67.87nm的平均粒径,如通过HR-TEM分析所证实的。St/Ag-SeNC对人大肠癌(HCT-116)和人乳腺癌(MCF-7)细胞系显示出有希望的抗癌活性,其IC50分别为21.37和19.98µg/ml,分别。同样,在低纳米复合材料浓度下观察到对红细胞的影响很小。同样,在浓度为2mg/ml时,纳米复合材料的H2O2清除活性最高(42.84%)。此外,表皮葡萄球菌(S.表皮)ATCC12,228和白色念珠菌(C.白色念珠菌)ATCC10,231是受高度影响的细菌和真菌菌株,其最低抑制浓度(MIC)为18.75和50µg/ml,分别。此外,St/Ag-SeNC对微生物生物膜的显着影响是浓度依赖性的。高生物膜抑制百分比,87.5%和68.05%,用表皮葡萄球菌和金黄色葡萄球菌(S.金黄色葡萄球菌)当暴露于1毫克/毫升和0.5毫克/毫升时,分别。
结论:生物合成的St/Ag-SeNC显示出优异的抗氧化活性,血液相容性,和抗增殖作用在低浓度。此外,它表现出有希望的抗菌和抗生物膜活性。
OBJECTIVE: Microbial cells capability to tolerate the effect of various antimicrobial classes represent a major worldwide health concern. The flexible and multi-components nanocomposites have enhanced physicochemical characters with several improved properties. Thus, different biological activities of biosynthesized starch/silver-selenium nanocomposite (St/Ag-Se NC) were assessed.
METHODS: The St/Ag-Se NC was biosynthesized using Cladosporium cladosporioides CBS 174.62 (C. cladosporioides) strain. The shape and average particle size were investigated using scanning electron microscope (SEM) and high-resolution transmission electron microscope (HR-TEM), respectively. On the other hand, the St/Ag-Se NC effect on two cancer cell lines and red blood cells (RBCs) was evaluated and its hydrogen peroxide (H2O2) scavenging effect was assessed. Moreover, its effects on various microbial species in both planktonic and biofilm growth forms were examined.
RESULTS: The St/Ag-Se NC was successfully biosynthesized with oval and spherical shape and a mean particle diameter of 67.87 nm as confirmed by the HR-TEM analysis. St/Ag-Se NC showed promising anticancer activity toward human colorectal carcinoma (HCT-116) and human breast cancer (MCF-7) cell lines where IC50 were 21.37 and 19.98 µg/ml, respectively. Similarly, little effect on RBCs was observed with low nanocomposite concentration. As well, the highest nanocomposite H2O2 scavenging activity (42.84%) was recorded at a concentration of 2 mg/ml. Additionally, Staphylococcus epidermidis (S. epidermidis) ATCC 12,228 and Candida albicans (C. albicans) ATCC 10,231 were the highly affected bacterial and fungal strains with minimum inhibitory concentrations (MICs) of 18.75 and 50 µg/ml, respectively. Moreover, the noticeable effect of St/Ag-Se NC on microbial biofilm was concentration dependent. A high biofilm suppression percentage, 87.5% and 68.05%, were recorded with S. epidermidis and Staphylococcus aureus (S. aureus) when exposed to 1 mg/ml and 0.5 mg/ml, respectively.
CONCLUSIONS: The biosynthesized St/Ag-Se NC showed excellent antioxidant activity, haemocompatibility, and anti-proliferative effect at low concentrations. Also, it exhibited promising antimicrobial and antibiofilm activities.