氧化损伤,活性氧(ROS)的过度积累加剧了,严重抑制作物生长和产量。在这里,我们开发了一种生物相容性纳米酶,六氰合铁酸钙纳米颗粒(CaHCFNPs),靶向ROS,减轻氧化损伤,促进植物生长过程中重金属离子的螯合。独特的,CaHCFNPs具有模拟六种天然酶的多面活性,涉及超氧化物歧化酶(SOD),过氧化氢酶(CAT),过氧化物酶(POD),谷胱甘肽过氧化物酶,硫醇过氧化物酶,和抗坏血酸过氧化物酶,这使得它们能够有效地中和一系列反应性物种,包括超氧化物阴离子,过氧化氢(H2O2),和羟基自由基。此外,CaHCFNPs促进钙离子与镉离子的交换过程,减少植物对重金属的吸收。重要的是,浓度为120μg/mL,CaHCFNPs缓解H2O2和氯化镉对拟南芥和番茄生长的抑制作用,增加SOD的活性,POD,CAT下降了46.2%,74.4%,和48.3%,分别,在受镉胁迫12天的拟南芥中。此外,非酶抗氧化剂还原型谷胱甘肽的水平也增加了72.4%。此外,CaHCFNPs促进抗氧化相关基因的表达,重金属解毒,养分运输,和抗压力。我们的发现揭示了配备纳米酶的纳米平台在减轻植物氧化应激方面的重要潜力。不仅可以调节作物的生长,而且可以大大提高产量和质量,预示着农业纳米技术的新时代。本文受版权保护。保留所有权利。
Oxidative damage, exacerbated by the excessive accumulation of reactive oxygen species (ROS), profoundly inhibits both crop growth and yield. Herein, a biocompatible nanozyme, calcium hexacyanoferrate nanoparticles (CaHCF NPs), targeting ROS is developed, to mitigate oxidative damage and sequestrate heavy metal ions during plant growth. Uniquely, CaHCF NPs feature multifaced enzyme-like activities, involving superoxide dismutase (SOD), catalase (CAT), peroxidase (POD), glutathione peroxidase, thiol peroxidase, and ascorbate peroxidase, which enable them to neutralize excessive ROS. Furthermore, CaHCF NPs promote calcium-cadmium exchange process, diminishing the uptake of heavy metals. Importantly, 120 µg mL-1 of CaHCF NPs alleviate the inhibitory effects of hydrogen peroxide and cadmium chloride on Arabidopsis and tomato. The activities of SOD, POD, and CAT increase by 46.2%, 74.4%, and 48.3%, respectively, meanwhile the glutathione level rises by 72.4% in Arabidopsis under cadmium stress. Moreover, CaHCF NPs boost the expression of genes associated with antioxidation, heavy metal detoxification, nutrient transport, and stress resistance. These findings unveil the significant potential of nanoplatforms equipped with nanozymes in alleviating oxidative stress in plants, which not only regulate crop growth but also substantially ameliorate yield and quality, heralding a new era in agricultural nanotechnology.