志贺氏菌被认为是主要的公共卫生问题,特别是发展中国家5岁以下的儿童。志贺氏菌的致病性是一个复杂的过程,涉及多个基因的相互作用,不稳定的毒力质粒以及染色体致病性岛。由于各种因素(包括毒力和抗生素抗性基因)与志贺氏菌病的严重程度和持续时间有关,在这篇文章中,我们的目的是评估HeLa细胞的侵袭是否受到志贺氏菌的影响。具有不同特性的分离株(包括血清群,毒力基因谱,和抗生素耐药性模式)从德黑兰的儿科患者中恢复,伊朗。10株不同血清群志贺氏菌(福氏志贺氏菌和宋内志贺氏菌)的细胞侵袭能力,基因谱分析(virA,sen,ipgD,ipaD,ipac,ipab,和ipaH),和抗生素抗性表型(氨苄青霉素,阿奇霉素,环丙沙星,萘啶酸,甲氧苄啶-磺胺甲恶唑,头孢克肟,头孢噻肟,米诺环素,和左氧氟沙星)通过HeLa细胞系中的噬斑形成测定法进行测量。结果表明,所有选定的志贺氏菌属。从儿科患者中回收的分离物能够侵入HeLa细胞,但斑块的总数和平均大小在分离株之间是不同的。与S.sonnei分离株相比,Flexneri分离株在HeLa细胞中的侵袭能力更高归因于特定毒力基因的存在;然而,这些毒力因子的作用还有待确定。
Shigella is considered a major public health concern, especially for children younger than 5 years of age in developing countries. The pathogenicity of Shigella is a complex process that involves the interplay of multiple genes located on a large, unstable virulence plasmid as well as chromosomal pathogenicity islands. Since various factors (including virulence and antibiotic resistance genes) are associated with the severity and duration of shigellosis, in this article, we aim to evaluate whether the invasion of HeLa cells is affected by Shigella spp. isolates with different characteristics (including serogroups, virulence gene profiles, and antibiotic resistance patterns) recovered from pediatric patients in Tehran, Iran. Cell invasion ability of 10 Shigella isolates with different serogroups (Shigella flexneri and Shigella sonnei), gene profiling (virA, sen, ipgD, ipaD, ipaC, ipaB, and ipaH), and antibiotic resistance phenotyping (ampicillin, azithromycin, ciprofloxacin, nalidixic acid, trimethoprim-sulfamethoxazole, cefixime, cefotaxime, minocycline, and levofloxacin) were measured by plaque-forming assay in HeLa cell lines. The results show that all the selected Shigella spp. isolates recovered from pediatric patients were able to invade HeLa cells, but the total number and average size of plaques were different between the isolates. The higher invasion ability of S. flexneri isolates in HeLa cells compared to S. sonnei isolates was attributed to the presence of particular virulence genes; however, the role of each of these virulence factors remains to be determined.