一品红的抗菌和细胞毒活性,馏分和纯化合物进行了评估。HCT116,HePG2和MCF7的细胞毒性测定显示出显着的IC50:分别针对HCT116和HePG2的非极性部分“正己烷”54.7和76.2µg/mL。抗菌结果表明,与总提取物(其中正丁醇和乙酸乙酯部分对测试的细菌菌株显示出显著的抗菌活性(P<0.05))相比,植物部分对测试的病原体显示出显著的潜力。从正己烷和正丁醇馏分中进行化合物的分离和结构测定。从正己烷馏分中,29-nor-环artan醇(1),lanost-8-en-3-ol(2a),环artan醇(2b)和kampferol-3,4'-二甲醚(3)被分离和结构鉴定,GC-MS初步鉴定了24个化合物。从极性正丁醇馏分中,4-O-β-D-吡喃葡萄糖基-2-羟基-6-甲氧基苯乙酮(4),4-O-α-L-鼠李糖基-(1→6)-β-D-吡喃葡萄糖基-2-羟基-6甲氧基-苯乙酮(5),分配槲皮素-3-O-吡喃葡萄糖苷(6)和异奥林(7)。通过核磁共振(NMR)谱和质谱确定所得化合物的结构。除化合物1和5外,所有报道的化合物均具有抗菌效率。化合物2对粪肠球菌(22±0.13mm)显示出选择性最高的活性,同时,4-O-β-D-吡喃葡萄糖基-2-羟基-6-甲氧基苯乙酮(4)对测试革兰氏阳性和革兰氏阴性细菌的MIC为1.15-1.88mg/mL。细胞毒性分析表明,kampferol-3,4'-二甲醚(3)表现出最高的活性,IC50值与阿霉素相匹配;对HCT116,HePG2和MCF7分别为111.46、42.67和44.90µM,然而,它对视网膜正常细胞系RPE1有毒。
Antibacterial and cytotoxic activities of Euphorbia balsamifera, fractions and pure compounds were evaluated. The cytotoxic assays for HCT116, HePG2 and MCF7 showed a significant IC50: 54.7 and 76.2 µg/mL of non-polar fraction \"n-hexane\" against HCT116 and HePG2, respectively. Antibacterial results revealed that plant fractions exhibited significant potential against the tested pathogens than the total extract where n-butanol and ethyl acetate fractions showed significant antibacterial activity (P < 0.05) against tested bacterial strains. Isolation and structure determination of compounds from n-hexane and n-butanol fractions were performed. From n-hexane fraction, 29-nor-cycloartanol (1), lanost-8-en-3-ol (2a), cycloartanol (2b) and kampferol-3,4\'-dimethyl ether (3) were isolated and structurally identified, along with 24 compounds were tentatively identified by GC-MS. From the polar n-butanol fraction, 4-O-β-D-glucopyranosyl-2-hydroxy-6-methoxyacetophenone (4), 4-O-α-L-rhamnosyl-(1 → 6)-β-D-glucopyranosyl-2-hydroxy-6methoxy-acetophenone (5), quercetin-3-O-glucopyranoside (6) and isoorientin (7) were assigned. Structures of the obtained compounds were determined by nuclear magnetic resonance (NMR) spectroscopy and mass spectrometry. Except compounds 1 and 5, all reported compounds announced antibacterial efficiency. Compound 2 showed selectively the highest activity against Enterococcus faecalis (22 ± 0.13 mm), meanwhile 4-O-β-D-glucopyranosyl-2-hydroxy-6-methoxyacetophenone (4) showed broadly the highest antibacterial activity with MIC of 1.15-1.88 mg/mL against the test Gram-positive and Gram-negative bacteria. Cytotoxic assays indicated that kampferol-3,4\'-dimethyl ether (3) exhibited the highest activity with matching IC50 values to doxorubicin; 111.46, 42.67 and 44.90 µM against HCT116, HePG2 and MCF7, respectively, however, it is toxic on retina normal cell line RPE1.