制备了一种新型的可见光驱动固定化酶,并将其用于水体中抗生素的完全矿化。固定化酶由生物炭(C/CN)和辣根过氧化物酶(HRP)修饰的氮化碳组成,建立具有协同作用的光酶偶联体系。其中,生物炭的引入不仅提高了酶的稳定性和负载能力,还提高了光催化剂的光吸收能力和载体分离效率。经过优化的固定化工艺,HRP的固体负荷可达251.03mg/g,和85.03%的酶活性在4℃储存18天后保留。在磺胺嘧啶(SDZ)降解实验中,60min内HRP/C3/CN降解率达到71.21%,远高于HRP(2.33%),CN(49.78%)和C3/CN(58.85%)。此外,在HRP/C/CN降解下,SDZ的总有机碳(TOC)去除率达到53.14%,分别是CN和C3/CN的6.47和1.74倍,分别。本研究表明,生物炭的引入对光-酶级联偶联体系具有重要意义,为HRP&g-C3N4体系在废水处理中的应用提供了新的策略。
A novel immobilized enzyme driven by visible light was prepared and used for complete mineralization of antibiotics in water bodies. The immobilized enzyme was composed of carbon nitride modified by biochar (C/CN) and horseradish peroxidase (
HRP), establishing the photo-enzyme coupling system with synergistic effect. Among them, the introduction of biochar not only improves the stability and loading capacity of the enzyme, but also improves the light absorption capacity and carrier separation efficiency of the photocatalyst. After the optimization of immobilization process, the solid load of
HRP could reach 251.03 mg/g, and 85.03 % enzyme activity was retained after 18 days of storage at 4 °C. In the sulfadiazine (SDZ) degradation experiment, the degradation rate of
HRP/C3/CN reached 71.21 % within 60 min, which was much higher than that of
HRP (2.33 %), CN (49.78 %) and C3/CN (58.85 %). In addition, under the degradation of
HRP/C/CN, the total organic carbon (TOC) removal rate of SDZ reached 53.14 %, which was 6.47 and 1.74 times that of CN and C3/CN, respectively. This study shows that the introduction of biochar is of great significance to the photo-enzyme cascade coupling system and provides a new strategy for the application of HRP&g-C3N4 system in wastewater treatment.