背景:血凝素-神经氨酸酶(HN)是新城疫病毒(NDV)的膜蛋白之一,在宿主病毒感染中起重要作用。因此,抗HN抗体对宿主保护自身免受NDV感染的能力至关重要,因为它们在病毒感染中具有关键功能。因此,HN已成为新城疫病毒疫苗开发中的候选蛋白。
方法:该报告使用了在伊朗分离的NDV的HN蛋白的全长序列(VIId亚型)。我们表征和鉴定与其他更普遍的NDV基因型相比的氨基酸取代,VII亚型和疫苗株。此外,生物信息学工具被用来确定三维结构,B细胞抗原表位的分子动力学模拟和预测。
结果:结果表明,我们的分离株的抗原区域与从不同地理地点分离的NDV的其他VII亚型相当。此外,通过使用我们的HN蛋白的最终3D结构,表位预测服务器提出氨基酸残基作为B细胞表位,这导致线性和构象抗原位点的引入。
结论:免疫信息学疫苗设计原理目前显示出巨大的潜力,可以快速,经济地开发新一代候选疫苗以根除传染性病毒,包括NDV。为了做到这一点,重点是可能被认为是抗原的残留物。
BACKGROUND: Haemagglutinin-neuraminidase (HN) is one of the membrane proteins of Newcastle disease virus (NDV) that plays a significant role during host viral infection. Therefore, antibodies against HN are vital for the host\'s ability to protect itself against NDV infection due to their critical functions in viral infection. As a result, HN has been a candidate protein in vaccine development against the Newcastle disease virus.
METHODS: This report used the full-length sequence of the HN protein of NDV isolated in Iran (VIId subgenotype). We characterize and identify amino acid substitutions in comparison to other more prevalent NDV genotypes, VII subgenotypes and vaccine strains. Furthermore, bioinformatics tools were applied to determine the three-dimensional structure, molecular dynamics simulation and prediction of B-cell antigenic epitopes.
RESULTS: The results showed that the antigenic regions of our isolate are quite comparable to the other VII subgenotypes of NDV isolated from different geographical places. Moreover, by employing the final 3D structure of our HN protein, the amino acid residues are proposed as a B-cell epitope by epitope prediction servers, which leads to the introduction of linear and conformational antigenic sites.
CONCLUSIONS: Immunoinformatic vaccine design principles currently exhibit tremendous potential for developing a new generation of candidate vaccines quickly and economically to eradicate infectious viruses, including the NDV. In order to accomplish this, focus is directed on residues that might be considered antigenic.