■这项研究旨在确定2014年至2020年海南ART失败患者的HIV-1亚型分布和HIV耐药性(HIVDR),中国。
■对海南ART失败的HIV/AIDS患者进行了为期7年的横断面研究。我们使用在线分型工具和最大似然系统发育树来确认具有pol序列的HIV亚型。使用斯坦福大学HIV耐药性数据库分析耐药性突变(DRMs)。
■共纳入307例HIV感染的ART失败患者,并获得241个可用的pol序列。在241名患者中,CRF01_AE占68.88%,其次是CRF07_BC(17.00%)和其他8种亚型(14.12%)。HIVDR的总体患病率为61.41%,和HIVDR对抗非核苷逆转录酶抑制剂(NNRTIs),核苷酸逆转录酶抑制剂(NRTIs),蛋白酶抑制剂(PIs)为59.75%,45.64%,和2.49%,分别。失业的病人,个体免疫力低下或机会性感染,2017年至2020年的样本增加了HIVDR的奇数比率。此外,HIVDR不太可能影响女性患者。NNRTI的常见DRM为K103N(21.99%)和Y181C(20.33%),M184V(28.21%)和K65R(19.09%)是抗NRTI的主要DRM。
■本研究强调了海南的HIV-1亚型多样性以及长期监测HIVDR的重要性。
UNASSIGNED: This study aimed to determine the HIV-1 subtype distribution and HIV drug resistance (HIVDR) in patients with ART failure from 2014 to 2020 in Hainan, China.
UNASSIGNED: A 7-year cross-sectional study was conducted among HIV/AIDS patients with ART failure in Hainan. We used online subtyping tools and the maximum likelihood phylogenetic tree to confirm the HIV subtypes with pol sequences. Drug resistance mutations (DRMs) were analyzed using the Stanford University HIV Drug Resistance Database.
UNASSIGNED: A total of 307 HIV-infected patients with ART failure were included, and 241 available pol sequences were obtained. Among 241 patients, CRF01_AE accounted for 68.88%, followed by CRF07_BC (17.00%) and eight other subtypes (14.12%). The overall prevalence of HIVDR was 61.41%, and the HIVDR against non-nucleoside reverse transcriptase inhibitors (NNRTIs), nucleotide reverse transcriptase inhibitors (NRTIs), and protease inhibitors (PIs) were 59.75%, 45.64%, and 2.49%, respectively. Unemployed patients, hypoimmunity or opportunistic infections in individuals, and samples from 2017 to 2020 increased the odd ratios of HIVDR. Also, HIVDR was less likely to affect female patients. The common DRMs to NNRTIs were K103N (21.99%) and Y181C (20.33%), and M184V (28.21%) and K65R (19.09%) were the main DRMs against NRTIs.
UNASSIGNED: The present study highlights the HIV-1 subtype diversity in Hainan and the importance of HIVDR surveillance over a long period.