一种肌动蛋白结合蛋白,凝溶胶蛋白(GSN)有两种亚型,血浆(pGSN)和胞质(cGSN)。pGSN和/或cGSN水平的变化已显示与几种疾病的发病机理有关。这项研究的目的是评估暴露于慢性持续缺氧(CSH)的动物中HIF-1的细胞内和细胞外GSNL水平的变化,除了细胞凋亡和细胞氧化还原状态。Sham组大鼠暴露于21%O2,低氧组大鼠分别暴露于13%和10%O2。血浆pGSN,HIF-1α,总抗氧化剂状态(TAS)和总氧化剂状态(TOS),和肺组织pGSN,HIF-1α,TAS,TOS,GSN水平,并测量凋亡细胞数。发现HIF-1α水平在组织中显著增加,尤其是在严重缺氧的人群中,在生化和组织学检查中。pGSN水平在血浆和组织中也显著降低。在cGSN中观察到组织的显著增加。据观察,虽然抗氧化活性在组织中占主导地位,氧化活性在血浆中占主导地位。特别是,HIF-1调节的缺氧反应对细胞存活非常重要。这项研究的结果表明,肺组织中cGSN和TAS水平的增加以及HIF-1α可以被认为是细胞保护机制的激活。
An actin binding protein, gelsolin (GSN) has two isoforms, plasma (pGSN) and cytosolic (cGSN). Changes in pGSN and/or cGSN levels have been shown to be associated with the pathogenesis of several diseases. The aim of this study was to evaluate changes in intracellular and extracellular GSNlevels with HIF-1 in animals exposed to chronic sustained hypoxia (CSH), in addition to apoptosis and the cellular redox status. The rats in the Sham group were exposed to 21% O2, and the rats in the hypoxia groups were exposed to 13 and 10% O2, respectively. Plasma pGSN, HIF-1α, Total Antioxidant Status (TAS) and Total Oxidant Status (TOS), and lung tissue pGSN, HIF-1α, TAS, TOS, GSN levels, and apoptotic cell numbers were measured. HIF-1α levels were found to increase significantly in the tissue, especially in the group with severe hypoxia, both in biochemical and histological examinations. pGSN levels were also significantly decreased in both plasma and tissue. Significant increases in tissue were observed in cGSN. It was observed that while the antioxidant activity was dominant in the tissue, the oxidant activity was dominant in the plasma. In particular, the response to hypoxia regulated by HIF-1 is very important for cellular survival. The results of this study showed that the increase in cGSN and TAS levels in the lung tissue together with HIF-1α can be considered as the activation of mechanisms for cellular protection.