本研究旨在研究南瓜(南瓜子)产生的氧化锌纳米颗粒(ZnONPs)的特征及其对人结肠癌细胞(HCT116)和非洲绿猴肾脏的选择性细胞毒性作用,Vero细胞.该研究还研究了ZnONPs的抗氧化活性。该研究还检查了ZnONPs的抗氧化性能。这是由于对ZnONP对正常细胞和HCT116细胞的比较细胞毒性作用的研究有限。利用傅里叶变换红外光谱(FTIR)对ZnONPs进行了表征,热重分析(TGA),透射电子显微镜/选定区域电子衍射(TEM/SAED),和扫描电子显微镜-能量色散X射线(SEM-EDX)测定化学指纹图谱,热稳定性,尺寸,元素的形态,分别。根据结果,发现南瓜中的ZnONP小于5μm,并且在自然界中会团聚。此外,ZnONP指纹图谱和SEM-EDX元素分析与以往文献相似,表明样品被证明是ZnONP。ZnONP在380°C的温度下也是稳定的,表明绿色材料在60-400°C下非常坚固。在两个不同的时间点(24和48小时)测量Vero细胞和HCT116细胞系的细胞活力,以使用AlamarBlue测定法评估ZnONP对这些细胞的细胞毒性作用。细胞毒性结果表明,ZnONPs不抑制Vero细胞,但对癌细胞有轻微毒性,剂量-反应曲线IC50=~409.7μg/mL。发现ZnONP的这种绿色合成对正常细胞无毒,但对HCT116细胞具有轻微的细胞毒性作用。一项理论研究使用分子对接来研究纳米颗粒与细胞周期蛋白依赖性激酶2(CDK2)的相互作用,探讨其抑制CDK2在癌症中的作用机制。应进行进一步研究以确定细胞毒性研究的合适浓度。此外,DPPH具有显著的抗氧化能力,IC50为142.857μg/mL。研究重点:南瓜籽提取物促进了快速,高产,和环境友好的ZnO纳米粒子的合成。分光光度分析用于研究光学性质,可扩展性,尺寸,形状,分散性,和ZnONPs的稳定性。评估了ZnONPs对Vero和HCT116细胞的细胞毒性,显示对Vero细胞和癌细胞的细胞毒性没有抑制作用。DPPH测定也用于研究生物纳米颗粒的抗氧化潜力。进行了分子对接研究,以研究ZnONPs与CDK2的相互作用,并探讨它们抑制CDK2在癌症中的作用的机制。
This study aimed to investigate the characterization of zinc oxide nanoparticles (ZnONPs) produced from Cucurbita pepo L. (pumpkin seeds) and their selective cytotoxic effectiveness on human colon cancer cells (HCT 116) and African Green Monkey Kidney, Vero cells. The study also investigated the antioxidant activity of ZnONPs. The study also examined ZnONPs\' antioxidant properties. This was motivated by the limited research on the comparative cytotoxic effects of ZnO NPs on normal and HCT116 cells. The ZnO NPs were characterized using Fourier-transform infrared spectroscopy (FTIR), Thermogravimetric Analysis (TGA), Transmission Electron Microscope/Selected Area Electron Diffraction (TEM/SAED), and Scanning Electron Microscope-Energy Dispersive X-ray (SEM-EDX) for determination of chemical fingerprinting, heat stability, size, and morphology of the elements, respectively. Based on the results, ZnO NPs from pumpkins were found to be less than 5 μm and agglomerates in nature. Furthermore, the ZnO NPs fingerprinting and SEM-EDX element analysis were similar to previous literature, suggesting the sample was proven as ZnO NPs. The ZnO NPs also stable at a temperature of 380°C indicating that the green material is quite robust at 60-400°C. The cell viability of Vero cells and HCT 116 cell line were measured at two different time points (24 and 48 h) to assess the cytotoxicity effects of ZnO NP on these cells using AlamarBlue assay. Cytotoxic results have shown that ZnO NPs did not inhibit Vero cells but were slightly toxic to cancer cells, with a dose-response curve IC50 = ~409.7 μg/mL. This green synthesis of ZnO NPs was found to be non-toxic to normal cells but has a slight cytotoxicity effect on HCT 116 cells. A theoretical study used molecular docking to investigate nanoparticle interaction with cyclin-dependent kinase 2 (CDK2), exploring its mechanism in inhibiting CDK2\'s role in cancer. Further study should be carried out to determine suitable concentrations for cytotoxicity studies. Additionally, DPPH has a significant antioxidant capacity, with an IC50 of 142.857 μg/mL. RESEARCH HIGHLIGHTS: Pumpkin seed extracts facilitated a rapid, high-yielding, and environmentally friendly synthesis of ZnO nanoparticles. Spectrophotometric analysis was used to investigate the optical properties, scalability, size, shape, dispersity, and stability of ZnO NPs. The cytotoxicity of ZnO NPs on Vero and HCT 116 cells was assessed, showing no inhibition of Vero cells and cytotoxicity of cancer cells. The DPPH assay was also used to investigate the antioxidant potential of biogenic nanoparticles. A molecular docking study was performed to investigate the interaction of ZnO NPs with CDK2 and to explore the mechanism by which they inhibit CDK2\'s role in cancer.