基于纳米材料的模拟酶,叫做纳米酶,近几十年来受到越来越多的关注;然而,它们缺乏生物相容性限制了生物医学应用,这可以通过表面改性来解决。在这项工作中,Co3O4纳米片被不同的官能团修饰,包括氨基,羧基,羟基,和巯基(NH2-Co3O4,COOH-Co3O4,OH-Co3O4和SH-Co3O4)。用XRD对改性后的Co3O4纳米片进行了表征,SEM,TEM,XPS,FTIR,TG,和Zeta潜力,验证不同功能组的修改是否成功。进一步研究了它们的模拟过氧化物酶性质和动力学过程,结果表明它们的催化活性顺序如下:NH2-Co3O4>SH-Co3O4>COOH-Co3O4>纯Co3O4>OH-Co3O4,修饰的Co3O4纳米酶的催化作用均遵循Michael-Menten动力学。结果表明,不同的官能团改变了它们的电子转移能力,并进一步影响了它们的催化活性。选择H2O2检测作为应用模子系统评价修饰的Co3O4纳米酶。与其他Co3O4纳米酶相比,用NH2-Co3O4纳米酶构建了0.01至40mmolL-1的较宽线性范围和1.5μmolL-1的检测下限。结果表明,通过官能团进行表面修饰是改善Co3O4纳米酶应用的有力策略。改性Co3O4纳米酶增强的催化活性和良好的生物相容性为相关应用提供了有价值的材料,如医学检测和抗氧化。
Nanomaterial-based mimetic enzymes, called nanozymes, received more and more attention in recent decades; however, their lack of biocompatibility limited the biomedical applications, which could be solved by surface modification. In this work, the Co3O4 nanoplates were modified by different functional groups, including the amino group, carboxyl group, hydroxyl group, and sulfhydryl group (NH2-Co3O4, COOH-Co3O4, OH-Co3O4, and SH-Co3O4). And the modified Co3O4 nanoplates were characterized by XRD, SEM, TEM, XPS, FTIR, TG, and the Zeta potential, verifying the successful modification of different functional groups. Their mimetic peroxidase properties and kinetics process were further studied and showed that the order of their catalytic activities was as follows: NH2-Co3O4 > SH-Co3O4 > COOH-Co3O4 > pure Co3O4 > OH-Co3O4, and the catalysis of modified Co3O4 nanozymes all followed Michaelis-Menten kinetics. The results indicated that the different functional groups changed their electron transfer ability, and further affected their catalytic activity. H2O2 detection was selected as an application model system to evaluate the modified Co3O4 nanozymes. Compared with other Co3O4 nanozymes, a wider linear range from 0.01 to 40 mmol L-1 and a lower detection limit of 1.5 μmol L-1 was constructed with NH2-Co3O4 nanozymes. The results suggested that surface modification by functional groups was a robust strategy to improve the application of Co3O4 nanozymes. The enhanced catalytic activity and good biocompatibility of modified Co3O4 nanozymes provided valuable materials for the relative application, such as medical detection and antioxidation.