胰腺腺癌是消化系统最常见的恶性肿瘤,被称为“癌症之王”,因为它被标记为恶性程度高,快速发展,生存不佳,预后不良。以前,据报道,碱性亮氨酸拉链和W2结构域1(BZW1)参与许多肿瘤的进展。然而,它在胰腺癌等消化系统肿瘤中的研究很少。探索与胰腺癌生存和预后相关的潜在生物标志物,为胰腺癌提供新的靶向治疗。我们起首剖析了BZW1在胰腺癌中的mRNA和卵白表达。然后,我们探讨了BZW1与胰腺癌患者生存预后和免疫浸润的相关性。最后,我们探索了BZW1相关基因富集分析,包括蛋白质-蛋白质相互作用网络,基因本体论功能富集分析,和京都百科全书的基因和基因组途径富集分析。胰腺癌组织中BZW1基因的mRNA和蛋白表达高于癌旁正常组织,而BZW1高表达的胰腺癌患者预后较差。此外,BZW1的表达与胰腺癌不同免疫细胞呈正相关或负相关,如CD4+T淋巴细胞,CD8+T淋巴细胞,B细胞,巨噬细胞,中性粒细胞,等。相关富集分析表明,我们获得了50个可用的实验确定的BZW1结合蛋白和100个与BZW1相关的靶向基因,相交基因是真核翻译终止因子1和鸟嘌呤核苷酸结合蛋白,α抑制活性多肽3.此外,BZW1与真核翻译终止因子1和鸟嘌呤核苷酸结合蛋白呈正相关,多肽3基因在胰腺癌中的α抑制活性。基因本体论富集分析表明,BZW1主要与mRNA加工等生物学过程有关,\"\"RNA剪接,翻译起始的“\”调节,“和”先天免疫反应的激活。“京都基因百科全书和基因组途径分析的结果进一步表明,BZW1可能通过“剪接体”和“核糖体”参与胰腺癌的发生。“BZW1基因可能是胰腺癌潜在的免疫治疗靶点和有希望的预后标志物。
Pancreatic adenocarcinoma is the most common malignant tumor of the digestive system and is called the \"king of cancer\" because it has been labeled with high malignancy, rapid progression, poor survival, and poor prognosis. Previously, it was reported that the basic leucine zipper and W2 domains 1 (BZW1) is involved in the progression of many tumors. However, its research in digestive system tumors such as pancreatic cancer is rarely studied. To explore potential biomarkers related to survival and prognosis of pancreatic cancer and provide a new targeted therapy for it. We first analyzed the mRNA and protein expression of BZW1 in pancreatic cancer. We then explored the correlation of BZW1 with survival prognosis and immune infiltration in pancreatic cancer patients. Finally, we explored BZW1-related gene enrichment analysis, including protein-protein interaction networks, gene ontology functional enrichment analysis, and Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis. The mRNA and protein expression of the BZW1 gene in pancreatic cancer tissues were higher than those in adjacent normal tissues, and pancreatic cancer patients with high BZW1 expression had a poor prognosis. In addition, the expression of BZW1 was positively or negatively correlated with different immune cells of pancreatic cancer, such as CD4 + T lymphocytes, CD8 + T lymphocytes, B cells, macrophages, neutrophils, etc. Correlation enrichment analysis showed that we obtained 50 available experimentally determined BZW1-binding proteins and 100 targeted genes related to BZW1, and the intersection genes were eukaryotic translation termination factor 1 and Guanine nucleotide binding protein, alpha inhibiting activity polypeptide 3. Moreover, there was a positive correlation between BZW1 and eukaryotic translation termination factor 1 and Guanine nucleotide binding protein, alpha inhibiting activity polypeptide 3 genes in pancreatic cancer. Gene ontology enrichment analysis showed BZW1 was mainly related to biological processes such as \"mRNA processing,\" \"RNA splicing,\" \"regulation of translational initiation,\" and \"activation of innate immune response.\" The results of Kyoto Encyclopedia of Genes and Genomes pathway analysis further indicated that BZW1 may be involved in pancreatic carcinogenesis through the \"spliceosome\" and \"ribosome.\" The BZW1 gene may be a potential immunotherapy target and a promising prognostic marker for pancreatic cancer.