在这项研究中,探讨生长激素变化对甲鱼相关基因及调控作用的影响,PCR扩增,实时荧光定量分析,采用酶切技术对生长抑素(SS)基因进行克隆和测序,生长激素受体(GHR),和中国的胰岛素样生长因子-1(IGF-I)序列。人生长激素对生长轴相关基因SSmRNA表达的影响,GHR,观察到不同性别的IGF-1。利用实时荧光定量PCR对海龟SS基因的研究表明,SS基因主要表达于神经系统和消化系统,在大脑中发现的最高表达,而GHR基因和IGF-I基因在中国的所有组织中均有表达。SS基因在大脑中表达,垂体,肝脏,胃,和肠,在大脑中表达最高,在肝脏中表达最低。在注射外源性生长激素的4周内,SS基因在两性大脑中的表达水平先升高后降低,呈现抛物线趋势,实验组的表达水平低于对照组。注射生长激素(GH)后,GHR基因在两性肝脏中的表达在第一周表现出显著的增加,在第二周下降到对照组水平,然后逐渐增加。最后,在3周和4周时,GHR基因的表达达到显着差异水平。就IGF-I基因而言,肝脏中表达水平的变化趋势与GHR基因相同。注射外源性生长激素后,尽管SS基因的表达增加了Reeves\'乌龟对GHR基因分泌的抑制作用,外源性生长激素可以替代GH和GHR的合成,加速乌龟的生长.实验表明,注射重组人生长激素影响SS的表达,GHR,和IGF-1基因,促进了里夫斯乌龟的生长.
In this study, to explore the effect of growth hormone changes on the related genes and regulatory roles of the turtle, PCR amplification, real-time fluorescence quantitative analysis, and enzyme cutting technology were used to clone and sequence the somatostatin (SS) gene, growth hormone receptor (GHR), and insulin-like growth factor-1 (IGF-I) sequence of Chinemys reevesii. The effects of human growth hormone on the mRNA expression of growth-axis-related genes SS, GHR, and IGF-1 in different sexes were observed. The study of the SS gene in turtles using real-time fluorescence quantitative PCR showed that the SS gene was mainly expressed in the nervous system and the digestive system, with the highest expression found in the brain, while the GHR gene and the IGF-I gene were expressed in all tissues of Chinemys reevesii. The SS gene was expressed in the brain, pituitary, liver, stomach, and intestine, with the highest expression in the brain and the lowest expression in the liver. Within 4 weeks of the injection of exogenous growth hormone, the expression level of the SS gene in the brain of both sexes first increased and then decreased, showing a parabolic trend, and the expression level of the experimental group was lower than that of the control group. After the injection of growth hormone (GH), the expression of the GHR gene in the liver of both sexes showed a significant increase in the first week, decreasing to the control group level in the second week, and then gradually increasing. Finally, a significant level of difference in the expression of the GHR gene was reached at 3 and 4 weeks. In terms of the IGF-I gene, the changing trend of the expression level in the liver was the same as that of the GHR gene. After the injection of exogenous growth hormone, although the expression of the SS gene increased the inhibition of the secretion of the GHR gene by the Reeves\' turtle, exogenous growth hormone could replace the synthesis of GH and GHR, accelerating the growth of the turtle. The experiments showed that the injection of recombinant human growth hormone affects the expression of SS, GHR, and IGF-1 genes, and promotes the growth of the Reeves\' turtle.