这项横断面研究旨在获得有关工人暴露于空气粉尘的知识,细菌和真菌物种,内毒素,生物膜的形成,以及钻井废物处理厂中的硫化氢(H2S)。总的来说,408个全班个人样本,66个工作区40个钻井废物,和参考(室外空气和海水)样品进行了分析。一些工人暴露于高水平的内毒素(207EU/m3),细菌(3.8×104菌落形成单位(CFU)/m3和9.8×104DNA拷贝/m3),或真菌(1.4×107CFU/m3和3,600拷贝/m3)。内毒素的暴露水平,细菌,H2S的峰取决于处理技术。与海水参考相比,所有类型的钻井废物都含有高浓度的细菌。在钻井废物盆地附近发现空气中细菌的浓度升高。总的来说,在工人接触中发现了116、146和112种不同的细菌,工作区,和钻井废物,分别。发现在钻井废物和空气(个人和工作区域)样品中发现的细菌物种重叠。在发现的细菌物种中,49被归类为人类病原体,如大肠杆菌,阴沟肠杆菌,和氧化克雷伯菌.总的来说,在工作环境中发现了44种真菌,其中6种被分类为人类病原体,如烟曲霉。总之,穿过钻井废物处理厂,钻井废物中存在人类病原体,工人的接触受到工厂处理的钻井废物的影响,这些废物暴露于内毒素和细菌。高暴露与学徒或化学工程师有关,和清洁工作,或污水,白天工作。
This cross-sectional study aims to obtain knowledge about workers\' exposure to airborne dust, bacterial and fungal species, endotoxin, biofilm formation, and hydrogen sulfide (H2S) in drilling waste treatment plants. In total, 408 full-shift personal samples, 66 work areas, 40 drilling waste, and reference (outdoor air and seawater) samples were analyzed. Some workers were exposed to high levels of endotoxin (207 EU/m3), bacteria (3.8 × 104 colony forming units (CFU)/m3 and 9.8 × 104 DNA copies/m3), or fungi (1.4 × 107 CFU/m3 and 3,600 copies/m3). The exposure levels to endotoxin, bacteria, and peaks of H2S were dependent on the treatment technique. All types of drilling waste contained large concentrations of bacteria compared to the seawater references. Elevated concentrations of airborne bacteria were found close to drilling waste basins. In total, 116, 146, and 112 different bacterial species were found in workers\' exposure, work areas, and the drilling waste, respectively. An overlap in bacterial species found in the drilling waste and air (personal and work area) samples was found. Of the bacterial species found, 49 are classified as human pathogens such as Escherichia coli, Enterobacter cloacae, and Klebsiella oxytoca. In total, 44 fungal species were found in the working environment, and 6 of these are classified as human pathogens such as Aspergillus fumigatus. In conclusion, across the drilling waste treatment plants, human pathogens were present in the drilling waste, and workers\' exposure was affected by the drilling waste treated at the plants with elevated exposure to endotoxin and bacteria. Elevated exposure was related to working as apprentices or chemical engineers, and working with cleaning, or slop water, and working in the daytime.