背景:龙舌豆是一种豆科植物,富含蛋白质,淀粉,纤维,和其他具有许多促进健康益处的微量营养素。它的果皮通常在废物部分也含有许多生物活性化合物。
方法:在当前的研究中,测试龙舌豆皮(DtbP)提取物是否存在植物化学物质。使用DtbP提取物生物合成Ag纳米颗粒。生成的DtbP银纳米粒子的表征是使用紫外-可见光谱分析完成的,FTIR光谱分析,SEM分析,EDX分析,XRD分析,zeta电位,和DLS研究。此外,通过使用细胞毒性(抑制HepG2癌细胞),对生物合成的Ag纳米颗粒的多生物活性进行比较评估,抗糖尿病药(α-葡萄糖苷酶抑制试验),和抗氧化剂(自由基清除)分析。
结果:DtbP-AgNPs的表征结果表明,AgNPs在24小时内合成。AgNPs几乎是球形的。AgNPs的生物学效应分析表明DtbP-AgNPs具有显著的细胞毒性,抗糖尿病药,和适度的抗氧化作用。本研究结果作为一个整体报告了利用豆类龙舌豆废皮的DtbP-AgNPs的生物合成,并评估了其多种生物活性。合成的DtbP-AgNPs可以作为制药行业的潜在候选药物,用于治疗与癌症有关的几种医学疾病的药物的配制。糖尿病,等。
BACKGROUND: The dragon tongue beans are a legume belonging to the Fabaceae family, are rich in protein, starch, fiber, and other micronutrients that have numerous health-promoting benefits. Its peel commonly the waste parts also contains lots of bioactive compounds.
METHODS: In the current research, dragon tongue bean peels (DtbP) extract is tested for the existence of phytochemicals. Ag nanoparticles are biosynthesized using DtbP extract. The generated DtbP silver nanoparticle characterization was accomplished using UV-Vis spectral analysis, FTIR spectral analysis, SEM analysis, EDX analysis, XRD analysis, zeta potential, and DLS study. Furthermore, comparative assessment on multi-biological activities of the biosynthesized Ag nanoparticles was accomplished by employing cytotoxicity (inhibition against HepG2 cancer cells), antidiabetic (α-glucosidase inhibition assay), and antioxidant (free-radical scavenging) analysis.
RESULTS: The characterization result of the DtbP-AgNPs demonstrated that the AgNPs synthesized within 24 h. The AgNPs are nearly spherical. The biological effect assay of AgNPs displayed that DtbP-AgNPs is having significant cytotoxicity, antidiabetic, and moderate antioxidant effect. This study results as a whole report the biosynthesis of DtbP-AgNPs utilizing the legume dragon tongue bean waste peel and assessment of their multiple biological activities. The synthesized DtbP-AgNPs could serve as a potential candidate in the pharmaceutical industries in the formulation of drugs for the treatment of several medical ailments concerning cancer, diabetes, etc.