■产后子宫内膜炎是一种普遍的生殖疾病,导致延长的开放期,不孕症,和其他并发症。虽然乳酸杆菌菌株可以通过减少子宫炎症来缓解这些疾病,由于缺乏直接的抗微生物作用和延长的治疗时间,它们的有效性是有限的。本研究旨在构建表达牛粒细胞-巨噬细胞集落刺激因子(GM-CSF)的重组约氏乳杆菌菌株,以评估其减轻产后子宫炎症的潜力。
■重组约氏乳杆菌菌株被工程化以表达牛GM-CSF,并通过阴道灌注施用于妊娠小鼠。产后子宫内膜炎是由大肠杆菌感染引起的,并评估了工程菌株的保护作用。炎症标志物(IL-6,IL-1β,TNF-α),髓过氧化物酶(MPO)活性,测量一氧化氮(NO)浓度。进行组织学检查以评估子宫形态和病理损害。
表达GM-CSF的重组约氏乳杆菌菌株显著降低了子宫中大肠杆菌感染诱导的炎症水平。IL-6,IL-1β的表达降低证明了这种降低。TNF-α,以及降低MPO活性和NO浓度。组织学检查显示,用重组GM-CSF菌株治疗的小鼠子宫形态得到改善,病理损伤减少。至关重要的是,重组菌株还通过降低炎症细胞因子水平对牛子宫内膜炎发挥有益作用,提示对临床牛子宫内膜炎的有益作用。
■表达GM-CSF的重组约氏乳杆菌通过减少炎性细胞因子在牛中表现出对产后子宫内膜炎的保护作用。结果表明,该工程菌株在预防产后子宫炎症的潜在临床应用。为相关疾病提供了一种新颖有效的保护选择,并提高了牛的繁殖效率。
UNASSIGNED: Postpartum endometritis is a prevalent reproductive disorder in bovines, leading to a prolonged open period, infertility, and other complications. While Lactobacillus strains can mitigate these conditions by reducing uterine inflammation, their effectiveness is limited due to a lack of direct anti microbial action and extended treatment duration. This study aimed to construct a recombinant Lactobacillus johnsonii strain expressing bovine Granulocyte-macrophage colony-stimulating factor (GM-CSF) to evaluate its potential in reducing postpartum uterine inflammation.
UNASSIGNED: The recombinant Lactobacillus johnsonii strain was engineered to express bovine GM-CSF and administered to pregnant mice via vaginal perfusion. Postpartum endometritis was induced using E. coli infection, and the protective effects of the engineered strain were assessed. Inflammatory markers (IL-6, IL-1β, TNF-α), myeloperoxidase (MPO) activity, and nitric oxide (NO) concentration were measured. Histological examination was performed to evaluate uterine morphology and pathological damage.
UNASSIGNED: The recombinant L. johnsonii strain expressing GM-CSF significantly reduced inflammation levels induced by E. coli infection in the uterus. This reduction was evidenced by decreased expression of IL-6, IL-1β, TNF-α, as well as reduced MPO activity and NO concentration. Histological examination revealed improved uterine morphology and reduced pathological damage in mice treated with the recombinant GM-CSF strain. Crucially, the recombinant strain also exerts beneficial effects on bovine endometritis by reducing levels of inflammatory cytokines, suggesting a beneficial effect on clinical bovine endometritis.
UNASSIGNED: The recombinant Lactobacillus johnsonii expressing GM-CSF demonstrated protective effects against postpartum endometritis in bovines by reducing inflammatory cytokines. The findings indicate the potential clinical application of this engineered strain in preventing postpartum uterine inflammation, offering a novel and effective protective option for related disorders and improving bovine reproductive efficiency.