背景:HCMV感染在人类中普遍存在。本研究旨在探讨人巨细胞病毒(HCMV)糖蛋白B(gB)基因型分布与HCMV,病毒载量,以及有症状婴儿的人口统计学和临床特征。还比较了血液和尿液样本中HCMV的检出率。
方法:分析了265例接受尿液HCMVDNA检测的婴儿的回顾性数据。通过定量荧光聚合酶链反应(PCR)和DNA测序检测91例HCMV阳性婴儿的病毒载量和gB基因型,分别。
结果:所有婴儿中HCMV感染的阳性率为46.04%(122/265),并且随着年龄的增长而迅速增加。在接受调查的91名婴儿中,肝功能异常是最常见的诊断(34/91,37.36%),其次是肺炎(21/91,23.07%)。gB的序列分析产生了两种遗传亚型:最普遍的gB3(47/91,51.65%),其次是gB1(44/91,48.35%)。gB3HCMV感染在0~2月龄婴儿中的发生率高于3~12月龄婴儿(χ2=4.38,p=0.0364)。数据显示,ALT和AST水平在抗HCMVIgM+IgG-组中显著高于抗HCMVIgM+IgG+和IgM-IgG+组中。此外,这项研究表明,血液中HCMVDNA的检出率明显低于尿液(χ2=6.7131,p=0.0096)。
结论:本研究介绍了婴儿HCMV感染状况及其与人口学特征和临床表现的关系。此外,这项研究表明,尿液PCR是检测HCMV感染的最合适方法。
HCMV infection is widespread in humans. This retrospective study aimed to explore the relationship between human cytomegalovirus (HCMV) glycoprotein B (gB) genotype distribution, viral load, and the demographic and clinical features of symptomatic infants. The detection rate of HCMV in blood and urine samples was also compared.
Retrospective data from 265 infants who underwent urine HCMV DNA testing were analyzed. The viral load and gB genotype were detected in 91 HCMV positive infants by quantitative fluorescence polymerase chain reaction (PCR) and DNA sequencing, respectively.
The positive rate of HCMV infection was 46.04% (122/265) in all infants, and increased rapidly with age. Among the 91 infants investigated, liver function abnormality was the most common diagnosis (34/91, 37.36%), followed by pneumonia (21/91, 23.07%). Sequence analysis of gB yielded two genetic subtypes: the most prevalent gB3 (47/91, 51.65%), followed by gB1 (44/91, 48.35%). The gB3 HCMV infection was more prevalent in infants aged 0-2 months than in infants aged 3-12 months (χ2 = 4.38, p = 0.0364). The data showed that ALT and AST levels were significantly higher in the anti-HCMV IgM+IgG- group than in the anti-HCMV IgM+IgG+ and IgM-IgG+ groups. In addition, this study showed that the detection rate of HCMV DNA in the blood was significantly lower than that in the urine (χ2 = 6.7131, p = 0.0096).
This study presents the HCMV infection status of infants and its relationship with their demographic characteristics and clinical manifestations. In addition, this study suggests that urinary PCR is the most appropriate assay for detecting HCMV infections.