小白蛋白表达(PV)神经元,根据钙结合蛋白小白蛋白的表达进行分类,在外侧a核(LHb)的功能和可塑性中起着至关重要的作用。本研究旨在通过收集有关小鼠LHbPV神经元异质性的信息来加深我们对LHb的理解。为了实现这一点,我们调查了LHbPV神经元中递质机制的比例,包括GABA,谷氨酸能,血清素能,胆碱能,多巴胺能神经递质标记物,使用转录组分析,mRNA原位杂交链反应,和免疫组织化学。LHbPV神经元包含三个子集:谷氨酸能,GABA能,谷氨酸能和GABA能机械双阳性。通过比较子集的百分比,我们发现LHb在地形上是前后组织的;GABA能和谷氨酸能PV神经元优先分布在前后LHb中,分别,揭示LHb的前后地形。此外,我们证实了侧GABA能PV神经元的中外侧地形图。这些发现表明,PV神经元在LHb的不同部分沿前后轴和中外侧轴发挥不同的作用。促进LHb的地形功能。这将是有趣的,以确定他们的地形是否有差异地参与与LHb相关的各种认知和动机过程,特别是后谷氨酸能PV神经元的参与。意义陈述表达小清蛋白(PV)的神经元是理解外侧a(LHb)神经回路的关键。小鼠LHbPV神经元有三个亚群:谷氨酸能,GABA能,谷氨酸能和GABA能机械双阳性,大多数是谷氨酸。在这里,我们证明了LHbPV神经元的这些子集在地形上是前后组织的,暗示LHb中的地形功能。
Parvalbumin-expressing (PV) neurons, classified by their expression of the calcium-binding protein parvalbumin, play crucial roles in the function and plasticity of the lateral habenular nucleus (LHb). This study aimed to deepen our understanding of the LHb by collecting information about the heterogeneity of LHb PV neurons in mice. To achieve this, we investigated the proportions of the transmitter machinery in LHb PV neurons, including GABAergic,
glutamatergic, serotonergic, cholinergic, and dopaminergic neurotransmitter markers, using transcriptome analysis, mRNA in situ hybridization chain reaction, and immunohistochemistry. LHb PV neurons comprise three subsets:
glutamatergic, GABAergic, and double-positive for
glutamatergic and GABAergic machinery. By comparing the percentages of the subsets, we found that the LHb was topographically organized anteroposteriorly; the GABAergic and
glutamatergic PV neurons were preferentially distributed in the anterior and posterior LHb, respectively, uncovering the anteroposterior topography of the LHb. In addition, we confirmed the mediolateral topography of lateral GABAergic PV neurons. These findings suggest that PV neurons play distinct roles in different parts of the LHb along the anteroposterior and mediolateral axes, facilitating the topographic function of the LHb. It would be interesting to determine whether their topography is differentially involved in various cognitive and motivational processes associated with the LHb, particularly the involvement of posterior
glutamatergic PV neurons.