目的:牙龈纤维瘤(GFs)是牙龈的纤维性病变,在文献中没有很好的定义。它们在组织学上与周围骨化性纤维瘤(POFs)相似,两者的特征都是致密纤维组织的细胞增殖,POF的不同之处在于它们表现出钙化灶。本研究旨在扩展GFs的免疫组织化学特征,并确认它们的成骨细胞表型。
方法:福尔马林固定,石蜡包埋的GFs,检查了牙龈的POF和纤维上皮息肉(FEP)。对特殊的富含AT的序列结合蛋白2(SATB2)进行免疫组织化学染色,runt相关转录因子2(RUNX2),骨钙蛋白和α-平滑肌肌动蛋白(αSMA)。通过光学显微镜评估切片,并基于染色细胞的百分比和染色强度分配免疫反应性评分(IRS)。
结果:GFs,POF,牙龈的FEP表达了成骨细胞标志物SATB2,RUNX2和骨钙蛋白。GFs和POFs表达αSMA,而牙龈的FEP则不表达。GFs和POFs具有相似的SATB2、RUNX2和αSMA染色模式。
结论:这些发现表明GFs和POFs表现出相似的免疫组织化学特征,并支持GFs是可能与POFs相关的成骨细胞病变的理论。
OBJECTIVE: Gingival fibromas (GFs) are fibrous lesions of the gingiva that are not well defined in the literature. They are histologically similar to peripheral ossifying fibromas (POFs), both being characterized as cellular proliferations of dense fibrous tissue, with POFs differing in that they demonstrate foci of calcification. This study aims to expand upon the immunohistochemical characterization of GFs, and to confirm their osteoblastic phenotype.
METHODS: Formalin fixed, paraffin embedded GFs, POFs and fibroepithelial polyps (FEPs) of the gingiva were examined. Immunohistochemical staining was performed for special AT-rich sequence binding protein 2 (SATB2), runt-related transcription factor 2 (RUNX2), osteocalcin and alpha-smooth muscle actin (αSMA). Sections were evaluated by light microscopy and the immunohistochemical staining patterns were assigned immunoreactive scores (IRS) based on percentage of stained cells and intensity of staining.
RESULTS: GFs, POFs, and FEPs of the gingiva expressed osteoblastic markers SATB2, RUNX2 and osteocalcin. GFs and POFs expressed αSMA while FEPs of the gingiva did not. GFs and POFs had similar staining patterns of SATB2, RUNX2 and αSMA.
CONCLUSIONS: These findings demonstrate that GFs and POFs exhibit a similar immunohistochemical profile, and supports a theory that GFs are osteoblastic lesions possibly related to POFs.