背景:越来越多的证据表明,在全球不同种族中,炎症与心房颤动(AF)的发病机制之间存在很强的关联。AF增加中风和心力衰竭的风险。尽管对IL-10反应进行了各种研究,临床证据有限,这证明了这些免疫调节剂在房颤中的作用。因此,这项研究旨在破译IL-10(-592A/C)多态性在术后AF(后OPAF)发展中的作用。
方法:本研究是为印度北部患者设计的。该研究包括90例房颤患者和126例窦性心律对照组,在心血管和胸外科接受手术。SGPGIMS,勒克瑙,印度。对DNA样品进行基因分型,以测定IL-10(-592A/C)基因中常见的单核苷酸多态性(SNP)。基于PCR的RFLP技术用于评估基因型频率。采用多变量logistic回归分析研究其他危险因素与房颤的关系。
结果:IL-10(-592A/C)基因型的分布(CC,AC,AA)为48.41%,47.61%,对照组为3.98%,对照组为41.11%,45.55%,13.34%的病例,分别(P=.0385)。病例组等位基因A频率明显高于对照组(36.11%vs27.77%,P=.0654)。与CC相比,在未经调整和调整的分析中,AA基因型的房颤风险均增加。
结论:这项研究表明,在印度北部患者中,IL-10(-592A/C)多态性可能与OP后AF的发生有显著关联。
BACKGROUND: There is an accumulating body of evidence indicating a strong association between inflammation and the pathogenesis of atrial fibrillation (AF) in different ethnicities across the globe. AF increases the risk of stroke and heart failure. Despite various researches on IL-10 response, there is limited clinical evidence present, which demonstrate a role of these immunity regulators in AF. Therefore, this study was designed to decipher the role of IL-10(-592A/C) polymorphism in the development of postoperative AF (post-OP AF).
METHODS: The study was designed for north Indian patients. The study included 90 patients with AF and 126 controls in sinus rhythm undergoing surgery at Department of Cardiovascular and thoracic surgery, SGPGIMS, Lucknow, India. DNA samples were genotyped for common single nucleotide polymorphism (SNP) in gene IL-10(-592A/C). The PCR-based RFLP technique was used to assess the genotype frequencies. The multivariable logistic regression analysis was performed to study the association of other risk factors with AF.
RESULTS: The distribution of IL-10(-592A/C) genotypes (CC, AC, and AA) was found to be 48.41%, 47.61%, and 3.98% in controls and 41.11%, 45.55%, and 13.34% in cases, respectively (P = .0385). The frequency of allele A in cases was significantly higher than the control group (36.11% vs 27.77%, P = .0654). Compared with CC, AA genotype had increased risk of AF in both unadjusted and adjusted analyses.
CONCLUSIONS: This study suggests that IL-10(-592A/C) polymorphism may have significant association with post-OP AF development in north Indian patients.