探讨动态增强磁共振成像(DCE-MRI)定量灌注直方图参数与肿瘤组织表皮生长因子受体(EGFR)表达的关系。血管内皮生长因子(VEGF)和EGFR基因突变与非小细胞肺癌(NSCLC)的关系。从2018年3月至2021年8月,共招募了44例已知NSCLC的连续患者。直方图参数(平均值,均匀性,偏斜度,能源,峰度,熵,每个(Ktrans,Kep,Ve,Vp,Fp)通过Omni动力学软件获得。免疫组化染色检测VEGF和EGFR蛋白的表达,PCR检测EGFR基因突变。进行相应的统计学检验,比较鳞状细胞癌(SCC)和腺癌(AC)的参数和蛋白表达。以及EGFR突变和野生型。采用相关性分析评价各参数与VEGF、EGFR蛋白表达的相关性。Fp(偏斜度,峰度,能量)在SCC和AC之间有统计学意义,ROC曲线下面积分别为0.733、0.700和0.675。VEGF在AC中的表达高于SCC。Fp(偏斜度,峰度,能量)与VEGF呈负相关(r=-0.527,-0.428,-0.342);Ktrans(Q50)与VEGF呈正相关(r=0.32);Kep(能量),Ktrans(偏斜度,峰度)与EGFR呈正相关(r=0.622,r=0.375,0.358),Kep的一些直方图参数,Ktrans(均匀性,熵)和Ve(峰度)与EGFR呈负相关(r=-0.312至-0.644)。一些灌注直方图参数在EGFR突变和野生型之间具有统计学意义,野生型高于突变型(P<0.05)。DCE-MRI定量灌注直方图参数对NSCLC的鉴别诊断有一定的价值,具有非侵入性评估细胞信号通路相关蛋白表达的潜力。
To explore the relationship between quantitative perfusion histogram parameters of dynamic contrast enhanced magnetic resonance imaging (DCE-MRI) with the expression of tumor tissue epidermal growth factor receptor (EGFR), vascular endothelial growth factor (VEGF) and EGFR gene mutations in non-small cell lung cancer (NSCLC). A total of 44 consecutive patients with known NSCLC were recruited from March 2018 to August 2021. Histogram parameters (mean, uniformity, skewness, energy, kurtosis, entropy, percentile) of each (Ktrans, Kep, Ve, Vp, Fp) were obtained by Omni Kinetics software. Immunohistochemistry staining was used in the detection of the expression of VEGF and EGFR protein, and the mutation of EGFR gene was detected by PCR. Corresponding statistical test was performed to compare the parameters and protein expression between squamous cell carcinoma (SCC) and adenocarcinoma (AC), as well as EGFR mutations and wild-type. Correlation analysis was used to evaluate the correlation between parameters with the expression of VEGF and EGFR protein. Fp (skewness, kurtosis, energy) were statistically significant between SCC and AC, and the area under the ROC curve were 0.733, 0.700 and 0.675, respectively. The expression of VEGF in AC was higher than in SCC. Fp (skewness, kurtosis, energy) were negatively correlated with VEGF (r = - 0.527, - 0.428, - 0.342); Ktrans (Q50) was positively correlated with VEGF (r = 0.32); Kep (energy), Ktrans (skewness, kurtosis) were positively correlated with EGFR (r = 0.622, r = 0.375, 0.358), some histogram parameters of Kep, Ktrans (uniformity, entropy) and Ve (kurtosis) were negatively correlated with EGFR (r = - 0.312 to - 0.644). Some perfusion histogram parameters were statistically significant between EGFR mutations and wild-type, they were higher in wild-type than mutated (P < 0.05). Quantitative perfusion histogram parameters of DCE-MRI have a certain value in the differential diagnosis of NSCLC, which have the potential to non-invasively evaluate the expression of cell signaling pathway-related protein.