本研究旨在探讨SAP2和GCN4在不同条件下白色念珠菌伊曲康唑(ITR)耐药中的作用,以及它们的相关性。共20株临床白色念珠菌,包括10个ITR抗性菌株和10个敏感菌株,被使用。然后,进行SAP2测序和GCN4测序,并测定了不同菌株的生物膜形成能力。最后,采用实时定量PCR技术检测浮游和生物膜条件下SAP2和GCN4在白色念珠菌中的表达,以及它们的相关性也进行了分析。没有错义突变和三个同义突变位点,包括T276A,G543A,和A675C,在SAP2测序中发现。GCN4测序显示一个错义突变位点(A106T(T36S))和六个同义突变位点(A147C,C426T,T513C,T576A,G624A和C732T)。白色念珠菌耐药菌株的生物膜形成能力显著高于敏感菌株(P<0.05)。此外,SAP2和GCN4在ITR抗性菌株中上调,在生物膜条件下,白色念珠菌均显著高于白色念珠菌。SAP2和GCN4的mRNA表达水平呈显著正相关。在浮游和生物膜条件下,在白色念珠菌的ITR抗性菌株中观察到SAP2和GCN4的较高表达水平,SAP2和GCN4mRNA表达呈正相关。
This study aimed to explore the roles of SAP2 and
GCN4 in itraconazole (ITR) resistance of C. albicans under different conditions, and their correlations. A total of 20 clinical strains of C. albicans, including 10 ITR resistant strains and 10 sensitive strains, were used. Then, SAP2 sequencing and
GCN4 sequencing were performed, and the biofilm formation ability of different C. albicans strains was determined. Finally, real-time quantitative PCR was used to measure the expression of SAP2 and
GCN4 in C. albicans under planktonic and biofilm conditions, as well as their correlation was also analyzed. No missense mutations and three synonymous mutation sites, including T276A, G543A, and A675C, were found in SAP2 sequencing. GCN4 sequencing showed one missense mutation site (A106T (T36S)) and six synonymous mutation sites (A147C, C426T, T513C, T576A, G624A and C732T). The biofilm formation ability of drug-resistant C. albicans strains was significantly higher than that of sensitive strains (P < 0.05). Additionally, SAP2 and
GCN4 were up-regulated in the ITR-resistant strains, and were both significantly higher in C. albicans under biofilm condition. The mRNA expression levels of SAP2 and
GCN4 had significantly positive correlation. The higher expression levels of SAP2 and GCN4 were observed in the ITR-resistant strains of C. albicans under planktonic and biofilm conditions, as well as there was a positive correlation between SAP2 and GCN4 mRNA expression.