目的:比较有无马胃腺疾病(EGGD)的马的小肠炎症和胃部炎症,我们评估了内窥镜,宏观,以及腺胃的微观发现和小肠的微观发现。
方法:36匹马。
方法:马接受了内窥镜检查,并对EGGD评分。安乐死后,收集胃并进行宏观评估。正常幽门粘膜,腺体病变,和小肠(十二指肠,空肠中部,和回肠)样品被收集和处理以进行显微镜检查。对细胞浸润进行评分。对轻度至中度淋巴浆细胞浸润的马的腹侧幽门和小肠进行免疫组织化学(CD3,CD20和Iba-1)。用Spearman相关系数评价EGGD分级与胃腺炎症的关系,以及腺胃中细胞浸润类型和严重程度的关系,十二指肠,空肠,和回肠.
结果:胃肠道炎症很常见,92%的人发现有胃炎浸润,83%的十二指肠炎症浸润,92%的空肠炎症浸润,92%的马匹有回肠炎症浸润.胃部疾病的内镜证据(充血或EGGD等级≥2/4)与十二指肠的存在或严重程度无关,空肠,或回肠炎症。胃淋巴浆细胞性炎症≥2级与十二指肠淋巴浆细胞性炎症≥2级相关。这是安乐死的马的方便样本。病史(包括驱虫史)不详。
结论:胃淋巴浆细胞性炎症与十二指肠淋巴浆细胞性炎症相关,但与远端小肠炎症无关。肠道炎症与内镜检查结果无关(充血或EGGD等级≥2/4)。
OBJECTIVE: To compare small intestinal inflammation with gastric inflammation in horses with and without equine gastric glandular disease (EGGD), we evaluated endoscopic, macroscopic, and microscopic findings of the glandular stomach and microscopic findings of the small intestine.
METHODS: 36 horses.
METHODS: Horses underwent endoscopy and were scored for EGGD. After euthanasia, stomachs were collected and macroscopically evaluated. Normal pyloric mucosa, glandular lesions, and small intestinal (duodenum, mid-jejunum, and ileum) samples were collected and processed for microscopic examination. Cellular infiltrate was scored. Immunohistochemistry (CD3, CD20, and Iba-1) was performed on the ventral pylorus and small intestine of horses with mild to moderate lymphoplasmacytic infiltrate. A Spearman\'s correlation coefficient was used to evaluate the relationship of EGGD grade with gastric glandular inflammation, and the relationships of cellular infiltrate type and severity among glandular stomach, duodenum, jejunum, and ileum.
RESULTS: Gastrointestinal inflammation was common, with gastric inflammatory infiltrate identified in 92%, duodenal inflammatory infiltrate in 83%, jejunal inflammatory infiltrate in 92%, and ileal inflammatory infiltrate in 92% of horses. Endoscopic evidence of gastric disease (hyperemia or EGGD grade ≥ 2/4) was not associated with the presence or severity of duodenal, jejunal, or ileal inflammation. Gastric lymphoplasmacytic inflammation grade ≥ 2 was associated with duodenal lymphoplasmacytic inflammation grade ≥ 2. This was a convenience sample of horses presenting for euthanasia. Medical history (including deworming history) was unknown.
CONCLUSIONS: Gastric lymphoplasmacytic inflammation is associated with duodenal lymphoplasmacytic inflammation but not more distal small intestinal inflammation. Intestinal inflammation is not associated with endoscopic findings (hyperemia or EGGD grade ≥ 2/4).