灵芝,在中国是一种重要的食用和药用蘑菇，仍然笼罩在关于控制活性成分积累和潜在蛋白质在其不同发育阶段表达的内在机制的有限理解中。因此，这项研究采用了代谢组学和蛋白质组学技术的精心整合，以审查整个生长期中灵芝代谢物积累和蛋白质表达的动态变化。代谢组学分析揭示了三萜类化合物的水平升高，类固醇,在蘑菇生长的出芽阶段（BS）和多酚化合物，具有突出的化合物，包括双戊酸和Gan，H,而我,除了关键的类固醇，如胆固醇和4,4-二甲基-5α-胆汁淤积-8,14,24-三烯-3β-醇。此外,营养素如多糖，黄酮类化合物，在子囊孢子的成熟期（FS），嘌呤表现出更高的存在。蛋白质组学研究证明了CYP450，HMGR，HMGS,和ERG蛋白家族，随着灵芝的进步，所有这些都表现出下降，除了ARE家族,显示了一个向上的轨迹。因此,建议BS作为灵芝的最佳收获期。这项调查为灵芝的整体开发提供了新的见解。
Ganoderma lucidum, renowned as an essential edible and medicinal mushroom in China, remains shrouded in limited understanding concerning the intrinsic mechanisms governing the accumulation of active components and potential protein expression across its diverse developmental stages. Accordingly, this study employed a meticulous integration of metabolomics and proteomics techniques to scrutinize the dynamic alterations in metabolite accumulation and protein expression in G. lucidum throughout its growth phases. The metabolomics analysis unveiled elevated levels of triterpenoids, steroids, and polyphenolic compounds during the budding stage (BS) of mushroom growth, with prominent compounds including Diplazium and Ganoderenic acids E, H, and I, alongside key steroids such as cholesterol and 4,4-dimethyl-5alpha-cholesta-8,14,24-trien-3beta-ol. Additionally, nutrients such as polysaccharides, flavonoids, and purines exhibited heightened presence during the maturation stage (FS) of ascospores. Proteomic scrutiny demonstrated the modulation of triterpenoid synthesis by the CYP450, HMGR, HMGS, and ERG protein families, all exhibiting a decline as G. lucidum progressed, except for the ARE family, which displayed an upward trajectory. Therefore, BS is recommended as the best harvesting period for G. lucidum. This investigation contributes novel insights into the holistic exploitation of G. lucidum.
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