铜配合物[Cu(L1H)ClO4](1)和[Cu(L2)NO3](2),与半乳糖氧化酶的金属位点有关,通过不同的光谱方法合成和表征。L1H2和L2H2[其中L1H2代表2,2'-((1E,1'E)(2,2'-(吡啶-2,6-二基)双(2-苯基肼-2-基-1-亚基)双(亚甲基))二酚和L2H2代表6,6'-((1E,1\'E)-(2,2\'-(吡啶-2,6-二基)双(2-苯基肼-2-基-1-亚基)双(亚甲基))双(2,4-二叔丁基苯酚),H代表可解离的质子]是五齿配体。这些配体提供吡啶基N,两个亚胺N,和两个非无辜的苯氧基和苯酚O供体,形成络合物1作为非自由基络合物,而络合物2是苯氧基自由基络合物。通过X射线晶体学鉴定了配合物1和2的分子结构。苯甲醇氧化进行了研究,并检查了9,10-二氢蒽向蒽的转化,以仔细检查H原子的提取反应。通过自激活的质粒DNA(pBR322)切割研究了复合物1和2的核酸酶活性。通过DFT计算研究了含配体的酚功能的非无辜性质。
Copper complexes [Cu(L1H)ClO4] (1) and [Cu(L2)NO3] (2), which are relevant to the metal site of the galactose oxidase enzyme, were synthesized and characterized by different spectroscopic methods. L1H2 and L2H2 [where L1H2 stands for 2,2\'-((1E,1\'E)(2,2\'-(pyridine-2,6-diyl)bis(2-phenylhydrazin-2-yl-1-ylidene))bis(methanylylidene))diphenol and L2H2 stands for 6,6\'-((1E,1\'E)-(2,2\'-(pyridine-2,6-diyl)bis(2-phenylhydrazin-2-yl-1-ylidene))bis(methanylylidene))bis(2,4-di-tert-butylphenol), H stands for dissociable proton] are pentadentate ligands. These ligands provide pyridyl N, two imine N, and two non-innocent phenoxyl and phenolato O donors, forming complex 1 as a non-radical complex, while complex 2 is a phenoxyl radical complex. The molecular structures of complexes 1 and 2 were authenticated by X-ray crystallography. Benzyl alcohol oxidation was investigated, and the conversion of 9,10-dihydroanthracene to anthracene was examined to scrutinize the H-atom abstraction reaction. Nuclease activity with complexes 1 and 2 was investigated by self-activated plasmid DNA (pBR322) cleavage. Non-innocent properties of the ligand-containing phenolato function were investigated by DFT calculations.