孤儿受体有未知的内源性配体,在不同的组织中表达,参与各种疾病,如糖尿病,高血压和癌症。我们研究了几种肿瘤组织中GPR21,GPR39,GPR135和GPR153孤儿受体的表达谱。子宫颈,乳房,皮肤,前列腺,使用RealtimePCR分析和星形细胞瘤组织的孤儿受体基因表达。GPR39在宫颈癌和前列腺癌组织中过度表达,GPR21和GPR135受体在宫颈中明显减少,乳房,皮肤,前列腺,和星形细胞瘤组织,与健康人成纤维细胞相比。总之,GPR21和GPR135受体基因在癌组织中表达降低。GPR39可能在宫颈癌和前列腺癌的发展和演变中起作用。这些数据表明,这些受体可能是新诊断方法的替代分子,以及针对肿瘤病理的新疗法的设计。
Orphan receptors have unknown endogenous ligands, are expressed in different tissues, and participate in various diseases such as diabetes, hypertension and cancer. We studied the expression profiles of GPR21, GPR39,
GPR135 and GPR153 orphan receptors in several tumour tissues. Cervical, breast, skin, prostate, and astrocytoma tissues were analysed for orphan receptor gene expression using Real time PCR analysis. GPR39 is over-expressed in cervical and prostate cancer tissues, and GPR21 and
GPR135 receptors are significantly decreased in cervical, breast, skin, prostate, and astrocytoma tissues, when compared with healthy human fibroblasts. In conclusion, GPR21 and
GPR135 receptor gene expression is reduced in cancerous tissues. GPR39 may have a role in the development and evolution of cervical and prostate cancer. These data suggest these receptors may be alternative molecules for new diagnostic approaches, and the design of novel therapeutics against oncological pathologies.