背景:研究人员已经研究了用于合成碳点的不同技术。这些技术包括电弧放电,激光烧蚀,氧化,水/溶剂热,和化学气相沉积。然而,这些技术受到一些限制,如气体带电粒子的利用,大电流,高温,强效氧化剂,非环保碳源,并产生不均匀的颗粒大小。因此,人们非常需要采用一种新技术,该技术结合了生物基碳采购和合成技术的环保方面。
结果:紫花苜蓿(苜蓿)衍生的N,已经通过微波辐射成功合成了S-CD。N,S-CD表现出强荧光(λex/em为320/420nm),荧光量子产率为2.2%,水溶性高。产生的N,S-CD用TEM表征,EDX,Zeta电位分析,IR,UV-可见光,和荧光光谱。产生的N的平均直径,S-CD为4.01±1.2nm,Zeta电位为-24.5±6.63mv。所生产的纳米传感器的稳定性也在宽pH范围内得到证实,长时间,并且存在不同的离子。合成的N,S-CD被用来量化抗菌药物,Nifuroxazide(NFZ),通过内部滤波效应机制实现荧光猝灭。该方法与NFZ浓度在1.0至30.0μM范围内呈线性关系。LOD和LOQ分别为0.16和0.49μM,分别。该方法用于量化模拟胃液(SGJ)中的NFZ,回收率为99.59±1.4,除了药物剂型外,AntinalCapsules®的回收率为98.75±0.61,Antinalsuspension®的回收率为100.63±1.54。方法验证按照ICH概述的标准进行。
■建议的方法主要集中在首次使用苜蓿,一种生态上可持续的多普勒CD来源,和通过微波辐射的经济有效的合成技术,其特点是能耗低,最小化反应时间,以及控制生产的CD大小的能力。这与全球对实施绿色分析化学原理的日益认可是一致的。
BACKGROUND: Researchers have investigated different techniques for synthesis of carbon dots. These techniques include Arc discharge, laser ablation, oxidation, water/solvothermal, and chemical vapor deposition. However, these techniques suffer from some limitations like the utilization of gaseous charged particles, high current, high temperature, potent oxidizing agents, non-environmentally friendly carbon sources, and the generation of uneven particle size. Therefore, there was a significant demand for the adoption of a new technology that combines the environmentally friendly aspects of both bio-based carbon sourcing and synthesis technique.
RESULTS: Medicago sativa L (alfalfa)-derived N, S-CDs have been successfully synthesized via microwave irradiation. The N,S-CDs exhibit strong fluorescence (λex/em of 320/420 nm) with fluorescence quantum yield of 2.2 % and high-water solubility. The produced N,S-CDs were characterized using TEM, EDX, Zeta potential analysis, IR, UV-Visible, and fluorescence spectroscopy. The average diameter of the produced N, S-CDs was 4.01 ± 1.2 nm, and the Zeta potential was -24.5 ± 6.63 mv. The stability of the produced nano sensors was also confirmed over wide pH range, long time, and in presence of different ions. The synthesized N, S-CDs were employed to quantify the antibacterial drug, nifuroxazide (NFZ), by fluorescence quenching via inner filter effect mechanism. The method was linear with NFZ concentration ranging from 1.0 to 30.0 μM. LOD and LOQ were 0.16 and 0.49 μM, respectively. The method was applied to quantify NFZ in simulated gastric juice (SGJ) with % recovery 99.59 ± 1.4 in addition to pharmaceutical dosage forms with % recovery 98.75 ± 0.61 for Antinal Capsules® and 100.63 ± 1.54 for Antinal suspension®. The Method validation was performed in compliance with the criteria outlined by ICH.
UNASSIGNED: The suggested approach primarily centers on the first-time use of alfalfa, an ecologically sustainable source of dopped-CDs, and a cost-effective synthesis technique via microwave irradiation, which is characterized by low energy consumption, minimized reaction time, and the ability to control the size of the produced CDs. This is in line with the growing global recognition of the implementation of green analytical chemistry principles.